Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding

文献类型: 外文期刊

第一作者: Zou, Xiuping

作者: Zou, Xiuping;Song, Erling;Peng, Aihong;He, Yongrui;Xu, Lanzhen;Lei, Tiangang;Yao, Lixiao;Chen, Shanchun;Zou, Xiuping;Peng, Aihong;He, Yongrui;Xu, Lanzhen;Lei, Tiangang;Yao, Lixiao;Chen, Shanchun

作者机构:

关键词: Pathogen-inducible promoter;Citrus canker;Xanthomonas axonopodis pv. citri (Xac);Wounding;GUS;Transgenic citrus

期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.711; 五年影响因子:2.73 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To improve the resistance of citrus to canker disease caused by Xanthomonas axonopodis pv. citri (Xac), it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated the functionality of PPP1 and hsr203J (Nicotiana tabacum L.) and gst1 (potato) pathogen-inducible promoters to drive expression of the beta-glucuronidase (GUS) reporter gene in transgenic citrus. The activities of these promoters in response to the Xac pathogen and wounding were determined quantitatively and qualitatively by fluorometric and histochemical GUS assays, and compared with that of the cauliflower mosaic virus (CaMV) 35S promoter. In citrus, the hsr203J promoter from tobacco was hardly activated by the Xac pathogen or wounding, whereas the PPP1 and gst1 promoters were rapidly and efficiently activated by both inducers. There was very little visible background expression from the PPP1 promoter, but a high level of background expression from the gst1 promoter. Because of its low background expression, the PPP1 promoter was more responsive to Xac and wounding than was the gst1 promoter. However, the gst1 promoter was more rapidly activated than the PPP1 promoter by Xac and wounding. The inducible activity of the two promoters was restricted to infection sites. Taken together, our results showed that the PPP1 promoter was the most efficient promoter among those evaluated in this study. Its strong responsiveness to Xac and wounding suggests that it would be a good candidate for expression of antibacterial transgenes specifically at infection sites to improve canker disease resistance in citrus.

分类号: Q942

  • 相关文献

[1]Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds. He, Yongrui,Chen, Shanchun,Peng, Aihong,Zou, Xiuping,Xu, Lanzheng,Lei, Tiangang,Liu, Xiaofeng,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Peng, Aihong,Zou, Xiuping,Xu, Lanzheng,Lei, Tiangang,Liu, Xiaofeng,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Peng, Aihong,Zou, Xiuping,Xu, Lanzheng,Lei, Tiangang,Liu, Xiaofeng,Yao, Lixiao. 2011

[2]Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus. Peng, Aihong,Chen, Shanchun,Lei, Tiangang,Xu, Lanzhen,He, Yongrui,Yao, Lixiao,Zou, Xiuping,Peng, Aihong,Chen, Shanchun,Lei, Tiangang,Xu, Lanzhen,He, Yongrui,Yao, Lixiao,Zou, Xiuping,Peng, Aihong,Chen, Shanchun,Lei, Tiangang,Xu, Lanzhen,He, Yongrui,Wu, Liu,Yao, Lixiao,Zou, Xiuping. 2017

[3]The Efficacy and Underlying Mechanism of Sulfone Derivatives Containing 1,3,4-oxadiazole on Citrus Canker. Li, Pei,Luo, Hui,Ma, Yuhua,Zhou, Junliang,Luo, Hui,Yan, Jiawen,Mao, Yongya,Wang, Zhuang.

[4]A putative 12-oxophytodienoate reductase gene CsOPR3 from Camellia sinensis, is involved in wound and herbivore infestation responses. Xin, Zhaojun,Zhang, Jin,Ge, Lingang,Lei, Shu,Han, Juanjuan,Zhang, Xin,Li, Xiwang,Sun, Xiaoling.

[5]Expression profiling of HbWRKY1, an ethephon-induced WRKY gene in latex from Hevea brasiliensis in responding to wounding and drought. Zhang, Quanqi,Zhu, Jiahong,Ni, Yanmei,Cai, Yuanbao,Zhang, Zhili,Zhang, Quanqi,Ni, Yanmei,Zhang, Zhili. 2012

[6]Effects of girdling on arbuscular mycorrhizal colonization and root hair development of litchi seedlings. Shu, Bo,Li, Weicai,Liu, Liqin,Wei, Yongzan,Shi, Shengyou.

[7]The Commelina Yellow Mottle Virus promoter is a strong promoter in vascular tissue of transgenic Gossypium hirsutum plants. Yuan, ZQ,Wu, JH,Wu, B,Li, YE,Chen, ZX,Li, SJ,Tian, YC. 2000

[8]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[9]The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter. Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,Han, Tianfu.

[10]Efficient regeneration and genetic transformation platform applicable to five Musa varieties. Liu, Juhua,Gao, Pengzhao,Zhang, Jing,Jia, Caihong,Zhang, Jianbin,Hu, Wei,Xu, Biyu,Jin, Zhiqiang,Sun, Peiguang,Wang, Jiashui,Jin, Zhiqiang,Sun, Xiuxiu. 2017

[11]Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Yang, LT,Ding, JY,Zhang, CM,Jia, JW,Weng, HB,Liu, WX,Zhang, DB. 2005

[12]A novel constitutive promoter and its downstream 5 ' UTR derived from cotton (Gossypium spp.) drive high-level gene expression in stem and leaf tissues. Sun Bao,Sun Guo-qing,Meng Zhi-gang,Zhang Rui,Guo San-dui. 2016

[13]GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots. Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,Han, Tianfu. 2014

[14]A 796 bp PsPR10 gene promoter fragment increased root-specific expression of the GUS reporter gene under the abiotic stresses and signal molecules in tobacco. Xu, Xiangbin,Guo, Sai,Chen, Kai,Liu, Junjun,Wang, Huizhong,Song, Hongmiao,Liu, Junjun,Guo, Longbiao,Qian, Qian.

[15]Isolation and characterization of Calcineurin B-like gene (PbCBL1) and its promoter in birch-leaf pear (Pyrus betulifolia Bunge). Xu, Y. Y.,Li, H.,Lin, J.,Li, X. G.,Chang, Y. H.. 2015

[16]Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco. Li, Ye,Liu, Xiaoqing,Zhou, Xiaojin,Yang, Wenzhu,Chen, Rumei,Li, Jie,Li, Suzhen,Chen, Guanyu.

[17]Multiple tissue-specific expression of rice seed-shattering gene SH4 regulated by its promoter pSH4. Yan, Huanxin,Ma, Li,Wang, Zhe,Lu, Bao-Rong,Lin, Zhimin,Su, Jun. 2015

[18]Expression of a novel bi-directional Brassica napus promoter in soybean. Chennareddy, Siva,Cicak, Toby,Clark, Lauren,Russell, Sean,Skokut, Michiyo,Beringer, Jeffrey,Jia, Yi,Gupta, Manju,Yang, Xiaozeng.

作者其他论文 更多>>