Ammonium Ion Exchanged Zeolite for Laser Desorption/Ionization Mass Spectrometry of Phosphorylated Peptides

文献类型: 外文期刊

第一作者: Yang, Mengrui

作者: Yang, Mengrui;Yang, Mengrui;Fujino, Tatsuya

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期刊名称:INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.885; 五年影响因子:2.52 )

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收录情况: SCI

摘要: alpha-Cyano-4-hydroxycinnamic acid (CHCA), an organic matrix molecule for matrix-assisted laser desorption/ionization mass spectrometry, was adsorbed to NH4+- type zeolite surface, and this new matrix was used for the detection of low-molecular-weight compounds. It was found that this matrix could simplify the mass spectrum in the low-molecular-weight region and prevent interference from fragments and alkali metal ion adducted species. CHCA adsorbed to NH4+-type ZSM5 zeolite (CHCA/NH(4)ZSM5) was used to measure atropine and aconitine, two toxic alkaloids in plants. In addition, CHCA/NH(4)ZSM5 enabled us to detect phosphorylated peptides; peaks of the protonated peptides had higher intensities than the peaks observed using CHCA only.

分类号: O65

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