Cryopreservation of axillary buds of grape (Vitis vinifera) in vitro plantlets

文献类型： 外文期刊

第一作者： Zhao, YH

作者： Zhao, YH;Wu, YJ;Engelmann, F;Zhou, MD

作者机构：

关键词： grapes;Vitis vinifera;cryopreservation;encapsulation-dehydration;cold-acclimation;freezing rate

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2001 年 22 卷 5 期

页码：

收录情况： SCI

摘要： Axillary buds sampled from in vitro plants of four grape varieties could withstand cryopreservation using the encapsulation-dehydration technique. Regrowth percentages ranged between 15 and 40%. Buds were encapsulated in alginate beads containing 0.5M sucrose, precultured at 5 degreesC on media containing daily increasing sucrose concentrations from 0.1 to 1.0M, then desiccated to 21% moisture content (fresh weight basis) and frozen slowly (prefreezing to -40 degreesC at 0.2 degreesC/min followed by immersion in liquid nitrogen). The physiological state of the in vitro mother-plants and the freezing procedure employed dramatically influenced the results. Regrowth after cryopreservation was achieved only when buds were sampled from mother-plants that had been kept without subculture for 3 to 4 months, An additional one-month period of cold-acclimation of mother-plants at 5 degreesC and a two-step freezing procedure improved growth recovery. The multiplication rate of shoots produced from cryopreserved buds was lower than that of controls during the first subcultures after thawing. However, the rooting ability of control and cryopreserved plantlets became similar from the fourth subculture onwards.

分类号：

  • 相关文献

[1]Cryopreservation of kiwi shoot tips. Wu, YJ,Zhao, YH,Engelmann, R,Zhou, MD. 2001

[2]Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 1999

[3]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[4]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[5]Changes in membrane-associated H+-ATPase activities and amounts in young grape plants during the cross adaptation to temperature stresses. Zhang, JH,Liu, YP,Pan, QH,Zhan, JC,Wang, XQ,Huang, WD.

[6]Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.. Liu, Jin-Ge,Zhang, Zhen,Qin, Qiu-Lin,Peng, Ri-He,Xiong, Ai-Sheng,Chen, Jian-Min,Xu, Fang,Zhu, Hong,Yao, Quan-Hong.

[7]Stereoselective Analysis and Dissipation of Propiconazole in Wheat, Grapes, and Soil by Supercritical Fluid Chromatography-Tandem Mass Spectrometry. Dong, Fengshou,Pan, Xinglu,Cheng, Youpu,Zhang, Yaofang,Sun, Shuhong,Li, Ning,Cui, Xinyi,Wang, Yuanhong,Zhang, Weilong,Li, Jing.

[8]Inheritance of anthocyanin content in the ripe berries of a tetraploid x diploid grape cross population. Liang, Zhenchang,Li, Shaohua,Liang, Zhenchang,Wu, Benhong,Liang, Zhenchang,Wu, Benhong,Sang, Min,Ma, Aihong,Zhao, Shengjian,Zhong, Gan-Yuan.

[9]Improved glyphosate resistance of 5-enolpyruvylshikimate-3-phosphate synthase from Vitis vinifera in transgenic Arabidopsis and rice by DNA shuffling. Tian, Yong-Sheng,Xu, Jing,Zhao, Wei,Fu, Xiao-Yan,Peng, Ri-He,Yao, Quan-Hong,Tian, Yong-Sheng,Tian, Yong-Sheng,Xing, Xiao-Juan.

[10]FLAVONOIDS ACCUMULATE IN CELL WALLS, MIDDLE LAMELLAE AND CALLOSE-RICH PAPILLAE DURING AN INCOMPATIBLE INTERACTION BETWEEN XANTHOMONAS CAMPESTRIS PV MALVACEARUM AND COTTON. Dai, GH,Nicole, M,Andary, C,Martinez, C,Bresson, E,Boher, B,Daniel, JF,Geiger, JP.

[11]First report of Neofusicoccum mangiferae associated with grapevine dieback in China. Dissanayake, Asha J.,Zhang, Wei,Chethana, Thilini,Zhang, Guozhen,Zhao, Wensheng,Dissanayake, Asha J.,Zhang, Wei,Li, Xinghong,Zhou, Ying,Chethana, Thilini,Yan, Jiye,Dissanayake, Asha J.,Chethana, Thilini,Chukeatirote, Ekachai,Hyde, Kevin D.,Dissanayake, Asha J.,Chethana, Thilini,Chukeatirote, Ekachai,Hyde, Kevin D..

[12]Somatic embryogenesis from immature zygotic embryos and monitoring the genetic fidelity of regenerated plants in grapevine. Yang, X. M.,An, L. Z.,Xiong, Y. C.,Zhang, J. P.,Li, Y.,Xu, S. J.,Yang, X. M..

[13]Ectopic expression of a Ve homolog VvVe gene from Vitis vinifera enhances defense response to Verticillium dahliae infection in tobacco. Tang, Juan,Lin, Jing,Chang, Youhong,Yang, Yuwen,Chen, Tianzi,Ling, Xitie,Zhang, Baolong,Chang, Youhong.

[14]The study of triploid progenies crossed between different ploidy grapes. Sun, Lei,Zhang, Guo-jun,Yan, Ai-ling,Xu, Hai-ying. 2011

[15]Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators. Cheng, Guo,Li, Qiang,Wang, Yu,Lan, Yi-Bin,Li, Si-Yu,Zhu, Yan-Rong,Song, Wen-Feng,Zhang, Xue,Cui, Xiao-Di,Wang, Jun,Wang, Yu,Lan, Yi-Bin,Li, Si-Yu,Wang, Jun,He, Yan-Nan,Chen, Wu,Sun, Run-Ze,Sun, Run-Ze,Cheng, Guo,Li, Qiang. 2017

[16]Gene expression profiling of rootstock '140Ru' and Vitis vinifera L. cv. 'Crimson Seedless' grape roots infected with grape phylloxera. Du, Yuan-Peng,Wang, Feng-Pan,Zhang, Shi-Zhong,Zhai, Heng,Jiang, En-Shun. 2014

[17]Characterization of Neopestalotiopsis, Pestalotiopsis and Truncatella species associated with grapevine trunk diseases in France. Maharachchikumbura, Sajeewa S. N.,Liu, Zuo-Yi,Maharachchikumbura, Sajeewa S. N.,Al-Sadi, Abdullah M.,Larignon, Philippe,Hyde, Kevin D.,Hyde, Kevin D.. 2016

[18]Extracts of Inula viscosa control downy mildew of grapes caused by Plasmopara viticola. Cohen, Y,Wang, WQ,Ben-Daniel, BH,Ben-Daniel, Y. 2006

[19]Dissecting the Variations of Ripening Progression and Flavonoid Metabolism in Grape Berries Grown under Double Cropping System. Wang, Yu,He, Lei,Yang, Xiao-Hui,He, Fei,Duan, Chang-Qing,Wang, Jun,Chen, Wei-Kai,Wang, Yu,He, Lei,Yang, Xiao-Hui,He, Fei,Duan, Chang-Qing,Wang, Jun,Bai, Xian-Jin,Cao, Mu-Ming,Cheng, Guo,Cao, Xiong-Jun,Guo, Rong-Rong. 2017

[20]The transport of C-14-salicylic acid in heat-stressed young Vitis vinifera plants. Wang, LJ,Huang, WD,Zhan, JC,Yu, FY.

作者其他论文 更多>>

微信小程序