Significant transcriptional changes in mature daughterVarroa destructormites during infestation of different developmental stages of honeybees

文献类型： 外文期刊

第一作者： Wu, Jiangli

作者： Wu, Jiangli;Tu, Yangyang;Getachew, Awraris;Xu, Shufa;Elsheikha, Hany M.;Zhou, Huaiyu;Zhou, Chunxue

作者机构：

关键词： Varroa destructor; Apis mellifera; transcriptomic analysis; infestation; neurotransmitter

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2020 年 76 卷 8 期

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收录情况： SCI

摘要： Background Varroa destructoris considered a major cause of honeybee (Apis mellifera) colony losses worldwide. AlthoughV. destructormites exhibit preference behavior for certain honeybee lifecycle stages, the mechanism underlying host finding and preference remains largely unknown. Results By using ade novotranscriptome assembly strategy, we sequenced the mature daughterV. destructormite transcriptome during infestation of different stages of honeybees (brood cells, newly emerged bees and adult bees). A total of 132 779 unigenes were obtained with an average length of 2745 bp and N50 of 5706 bp. About 63.1% of the transcriptome could be annotated based on sequence homology to the predatory miteMetaseiulus occidentalisproteins. Expression analysis revealed that mature daughter mites had distinct transcriptome profiles after infestation of different honeybee stages, and that the majority of the differentially expressed genes (DEGs) of mite infesting adult honeybees were down-regulated compared to that infesting the sealed brood cells. Gene ontology and KEGG pathway enrichment analyses showed that a large number of DEGs were involved in cellular process and metabolic process, suggesting thatVarroamites undergo metabolic adjustment to accommodate the cellular, molecular and/or immune response of the honeybees. Interestingly, in adult honeybees, some mite DEGs involved in neurotransmitter biosynthesis and transport were identified and their levels of expression were validated by quantitative polymerase chain reaction (qPCR). Conclusion These results provide evidence for transcriptional reprogramming in mature daughterVarroamites during infestation of honeybees, which may be relevant to understanding the mechanism underpinning adaptation and preference behavior of these mites for honeybees. (c) 2020 Society of Chemical Industry

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