MaGA20ox2f, an OsSD1 homolog, regulates flowering time and fruit yield in banana

文献类型： 外文期刊

第一作者： Zhao, Wei

作者： Zhao, Wei;Hu, Chunhua;Deng, Guiming;Sheng, Ou;Bi, Fangcheng;He, Weidi;Dou, Tongxin;Dong, Tao;Li, Chunyu;Liu, Siwen;Gao, Huijun;Yi, Ganjun;Yang, Qiaosong;Zhao, Wei;Hu, Chunhua;Deng, Guiming;Sheng, Ou;Bi, Fangcheng;He, Weidi;Dou, Tongxin;Dong, Tao;Li, Chunyu;Liu, Siwen;Gao, Huijun;Yi, Ganjun;Yang, Qiaosong;Zhao, Wei;Li, Chunlong;Sun, Xiaoxuan;Wu, Shaoping;Wu, Shuofan;Huo, Heqiang

作者机构：

关键词： GA20 oxidase2; Gibberellin; Flowering time; Fruit yield; Banana

期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）

ISSN： 1380-3743

年卷期： 2025 年 45 卷 1 期

页码：

收录情况： SCI

摘要： Previous studies illustrated that two banana GA20 oxidase2 (MaGA20ox2) genes, Ma04g15900 and Ma08g32850, are implicated in controlling banana growth and development; however, the biological function of each gene remains unknown. Ma04g15900 protein (termed MaGA20ox2f in this article) is the closest homolog to the Rice SD1 (encoded by 'green revolution gene', OsSD1) in the banana genome. The expression of MaGA20ox2f is confined to leaves, peduncles, fruit peels, and pulp. Knockout of MaGA20ox2f by CRISPR/Cas9 led to late flowering and low-yielding phenotypes. The flowering time of Delta MaGA20ox2f #1 and triangle MaGA20ox2f #2 lines was delayed approximately by 61 and 58 days, respectively, while fruit yield decreased by 81.13% and 76.23% compared to wild type under normal conditions. The endogenous levels of downstream products of GA20 oxidase, GA15 and GA20, were significantly reduced in triangle MaGA20ox2f mutant shoots and fruits, but bioactive GA1 was only significantly reduced in the mutant fruits. Quantitative proteomics analysis identified 118 up-regulated proteins and 309 down-regulated proteins in both Delta MaGA20ox2f #1 and triangle MaGA20ox2f #2 lines, compared to wild type, with the down-regulated proteins primarily associated with photosynthesis, porphyrin and chlorophyll metabolism. The decreased chlorophyll contents in Delta MaGA20ox2f #1 and triangle MaGA20ox2f #2 lines corroborated the findings of the proteomics data. We propose that photosynthesis inhibition caused by lower chlorophyll contents in Delta MaGA20ox2f mutant leaves and GA1 deficiency in Delta MaGA20ox2f mutant fruits may be the two critical reasons contributing to the late flowering and low-yielding phenotypes of Delta MaGA20ox2f mutants.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序