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Discovery of Sex-Determining Genes in Portunus trituberculatus: A Comparison of Male and Female Transcriptomes During Early Developmental Stages

文献类型： 外文期刊

作者： Zhang, Wen 1 ; Lv, Jianjian 1 ; Lan, Weikang 1 ; Gao, Baoquan 1 ; Liu, Ping 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

3.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian, Peoples R China

关键词： Portunus trituberculatus; transcriptome; sex-determining; Dmrt; PtDMY

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：5.247； 五年影响因子：5.72 ）

ISSN：

年卷期： 2022 年 8 卷

页码：

收录情况： SCI

摘要： Portunus trituberculatus is one of the main mariculture crabs of high economic value. To identify genes involved in sex determination, we first performed sex-specific transcriptome sequencing at six larval development stages using a DNA/RNA co-extraction method. A total of 907,952,938 and 828,774,880 reads were obtained from female and male crabs, respectively. 2,379 differentially expressed genes (DEGs) were found between females and males, and were mainly enriched in DNA replication, folate biosynthesis, and retinol metabolism pathways. Furthermore, transcription patterns of genes in the sex-determining region (SD) were analyzed based on the transcriptome data, and one Dmrt gene (PtDMY) was found to be exclusively expressed in males during early developmental stages. Notably, some known sex-related genes, including IAG, Dmrt11E, DmrtB1, and DmrtC2 were significantly down-regulated after knocking down PtDMY. Our results suggested that PtDMY is involved in sex determination and may be one of the key upstream regulators of the sex determination pathway. In addition, the massive volume of transcriptome data obtained in this study provided an important basis for the systematic study of sex determination mechanisms in P. trituberculatus.

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