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Immunoregulatory mechanisms and cross-kingdom bacteriostatic effects of microRNAs in crustacean

文献类型： 外文期刊

作者： Sun, Dongfang 1 ; Zhou, Xianfa 1 ; Su, Yichen 1 ; Gao, Baoquan 1 ; Liu, Ping 1 ; Lv, Jianjian 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Natl Key Lab Mariculture Biobreeding & Sustainable, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China

关键词： Cross-species; Crustacean; Immunoregulatory; miRNA; Vibrio parahaemolyticus

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 311 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are crucial regulators of gene expression, which contribute to immune response regulation in various organisms, including crustaceans. To investigate the immunoregulatory roles of miRNAs in Portunus trituberculatus, a comparative miRNAomic analysis of Vibrio parahaemolyticus infection was carried out. Through comparative miRNAomic analysis, we identified 17 differentially expressed miRNAs (DE-miRNAs), of which 12 were upregulated. Subsequently, miRNA-mRNA regulatory network analysis revealed that the DE-miRNAs were enriched in immune-related signaling pathways. Within the miRNA-mRNA regulatory network, miRNA novel0045 was identified as a crucial regulator of the tumor necrosis factor (TNF) pathway via targeting the TNF receptor-associated factor 6 gene. This result was corroborated by our RNA interference assay, confirming the significance of miRNA novel0045 in resistance to V. parahaemolyticus infection. Moreover, miRNA novel0294 was noted to possess cross-kingdom regulatory potential, translocating into bacterial cells and directly inhibiting V. parahaemolyticus proliferation. We validated this finding through fluorescence labeling and confocal microscopy, confirming effective internalization and presence of miRNA within bacterial. These results expand the current understanding of miRNA-mediated immune responses in crustaceans, highlighting the roles of miRNAs in host immune defense and cross-kingdom regulatory function in bacterial infection suppression, and have potential implications in the development of RNA-based antimicrobial strategies.

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