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Molecular characterization of TNF-ss and IFN-gamma in yellowfin seabream (Acanthopagrus latus, Hottuyn, 1782) and their immune responses to density stress during transport

文献类型： 外文期刊

作者： Liang, Yu 1 ; Zhu, Ke-Cheng 1 ; You, Ying-Zhe 2 ; Guo, Hua-Yang 1 ; Chen, He-Dong 2 ; Liu, Bao-Suo 1 ; Zhang, Nan 1 ; Dai, Yan-Bin 2 ; Zeng, Fan-Rong 2 ; Lin, Huan-Yang 3 ; Zhang, Dian-Chang 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China

2.Zhangzhou Aquat Technol Promot Stn, Zhangzhou 363000, Fujian, Peoples R China

3.Zhangzhou Marine Environm Monitoring Ctr, Zhangzhou 363000, Fujian, Peoples R China

4.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou 510300, Guangdong, Peoples R China

5.Sanya Trop Fisheries Res Inst, Sanya 572018, Peoples R China

6.231 Xingang Rd West, Guangzhou 510300, Peoples R China

关键词： Acanthopagrus latus; TNF-ss; IFN-gamma; Density stress

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0145-305X

年卷期： 2023 年 147 卷

页码：

收录情况： SCI

摘要： The inflammatory cytokines TNF-ss and IFN-gamma are important mediators of the vertebrate inflammatory response and coordinators of the immune system in regard to NF-kappa B signalling pathways. In this study, the TNF-ss and IFN-gamma genes of yellowfin seabream, Acanthopagrus latus were identified, and the multiple sequence alignments, evolutionary relationships and gene expressions of the two genes were also determined. AlTNF-ss contained a 762 bp open reading frame (ORF) encoding 253 amino acids, while AlIFN-gamma contained a 582 bp ORF encoding 193 amino acids. An amino-acid sequence alignment analysis showed that these proteins have highly conserved transmembrane structural domains among teleosts. Moreover, AlTNF-ss has a close affinity with TNF-ss of yellowfin seabream while AlIFN-gamma has a high evolutionary correlation with A. regius and Sparus aurata. In addition, the mRNAs of AlTNF-ss and AlIFN-gamma are widely expressed in various tissues. AlTNF-ss is highly expressed in gill and intestinal tissues, and the mRNA levels of AlIFN-gamma are higher in spleen, skin, and gill tissues than in other tissues. Under transportation density stress, the mRNA level of AlTNF-ss was significantly elevated in the intestine of the high-density group, while AlTNF-ss transcription in the gills did not vary significantly among the density groups. Furthermore, AlIFN-gamma expression was increased in liver, intestinal, and gill tissues under high transportation density. The results of this study show that TNF-ss and IFN-gamma expression in yellowfin seabream is greatly affected by density stress. The density of 125 per bag for 4-5 cm fry or 1200 per bag for 1-2 cm fry is most suitable for the transportation of live fish. These results might provide a reference for further studies on the immunomodulatory response process and auxiliary function of immune stress of TNF and IFN genes in fish under density stress.

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