Characterization and potential function of 7-dehydrocholesterol reductase (dhcr7) and lathosterol 5-desaturase (sc5d) in Cynoglossus semilaevis sexual size dimorphism
文献类型： 外文期刊
作者： Wang, Jialin 1 ; Shi, Rui 1 ; Yang, Qian 1 ; Chen, Zhangfan 1 ; Wang, Jiacheng 1 ; Gong, Zhihong 1 ; Chen, Songlin 1 ; Wang, Na 1 ;
作者机构： 1.Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Pilot Natl Lab Marine Sci & Technol, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Ocean Univ China, Coll Marine Life, Qingdao 266100, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
5.Shandong Key Lab Marine Fisheries Biotechnol & Gen, Qingdao 266071, Peoples R China
关键词： Chinese tongue sole ( Cynoglossus semilaevis ); 7-dehydrocholesterol reductase ( dhcr7 ); lathosterol 5-desaturase ( sc5d ); Promoter activity; siRNA; Protein subcellular localization
期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）
ISSN： 0378-1119
年卷期： 2023 年 853 卷
页码：
收录情况： SCI
摘要： The typical sexual size dimorphism (SSD) phenomenon of Chinese tongue sole (Cynoglossus semilaevis) seriously restricts the sustainable development of the fishing industry. Previous transcriptome analysis has found a close relationship between the steroid biosynthesis and C. semilaevis SSD. The 7-dehydrocholesterol reductase (dhcr7) and lathosterol 5-desaturase (sc5d) are two genes in the steroid biosynthesis pathway, playing important roles in lipid synthesis, cellular metabolism, and growth. The present study assessed their roles in the mechanism of C. semilaevis SSD. The quantitative polymerase chain reaction (qPCR) results showed that C. semilaevis dhcr7 was mainly expressed in female livers, and C. semilaevis sc5d was highly expressed in female livers and gonads. Dualluciferase experiment showed that dhcr7 and sc5d promoters had strong transcriptional activity. The transcription factors E2F transcription factor 1 (E2F1), and CCAAT enhancer binding protein alpha (C/EBP alpha) significantly regulated the transcriptional activity of dhcr7 and sc5d promoters, respectively. Furthermore, small interfering RNA (siRNA) knockdown results showed that expression levels of several genes [SREBF chaperone (scap), membrane-bound transcription factor peptidase, site 1 (mbtps1), fatty acid synthase (fasn), sonic hedgehog (shh), bone morphogenetic protein 2b (bmp2b) and AKT serine/threonine kinase 1 (akt1)] were suppressed. Protein subcellular localization results indicated that Dhcr7 and Sc5d were both specifically distributed in the cytoplasm, with colocalization been observed. The present study provides evidence that dhcr7 and sc5d might regulate C. semilaevis sexual size dimorphism by involving in energy homeostasis and cell cycle, or by affecting PI3K-Akt and Shh signaling pathways. The detailed roles of these steroid biosynthesis genes regulating C. semilaevis SSD needed more information.
- 相关文献
作者其他论文 更多>>
-
Bile acids help maintain immune homeostasis and enhance antioxidant capacity upon Vibrio infection in leopard coral grouper
作者：Zhang, Junwei;Yang, Bowen;Shi, Meng;Chen, Songlin;Zhou, Qian;Zhang, Junwei;Zhang, Jiquan;Wang, Shengpeng
关键词：Plectropomus leopardus; BAs; Vibrio harveyi infection; Transcriptomics; Metabolomics
-
Implications of seawater characteristics on dissolved heavy metals in near-shore surface waters of the Yellow Sea
作者：Li, Minghao;Ding, Dongsheng;Xu, Yong;Yang, Qian;Qu, Keming;Cui, Zhengguo;Wei, Yuqiu;Li, Minghao;Ding, Dongsheng;Xu, Yong;Yang, Qian;Qu, Keming;Cui, Zhengguo;Wei, Yuqiu;Sun, Jun
关键词：Heavy metals; Temperature; Salinity; Dissolved oxygen; Yellow Sea
-
A QTL associated with vibriosis resistance highlights the significant role of jam2a gene in tongue sole (Cynoglossus semilaevis)
作者：Li, Yangzhen;Chi, Yong;Yan, Xu;Hu, Yuanri;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Li, Zhongming;Jia, Lei
关键词：Genome-wide association; Quantitative trait locus; Transcriptome analysis; Heritability; Immune response
-
Orchestrated immune responses to Mycobacterium marinum natural infection in tongue sole ( Cynoglossus semilaevis)
作者：Li, Yangzhen;Yan, Xu;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Song, Limin;Robeldo, Diego;Mukiibi, Robert;Robeldo, Diego;Mukiibi, Robert;Robeldo, Diego;Mukiibi, Robert
关键词：Mycobacteriosis; Bacterial infection; RNA-Seq; Transcriptome analysis; Disease resistance
-
Genome-wide association study and transcriptome analysis provide the SNPs and molecular insights into the visceral nodular disease tolerance in half-smooth tongue sole (Cynoglossus semilaevis)
作者：Chang, Mengyang;Shi, Kunpeng;Xia, Jinqi;Zhang, Ruixue;Li, Yishuai;Zang, Shaoqing;Tan, Suxu;Wang, Wenwen;Sha, Zhenxia;Chen, Songlin;Sha, Zhenxia;Chen, Songlin
关键词：Cynoglossus semilaevis; Visceral nodular disease; GWAS; RNA-seq; Disease tolerance; Multi omics
-
A newly developed 20 K SNP array reveals QTLs for disease resistance to Cryptocaryon irritans in tiger pufferfish (Takifugu rubripes)
作者：Li, Yangzhen;Fraslin, Clemence;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Li, Yangzhen;Li, Weidong;Li, Weiguo;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Houston, Ross D.;Robledo, Diego
关键词：SNP array; Heritability; Genomic prediction; GWAS; QTL; Selective breeding
-
SHP-1 interacts with NFκB1 to inhibit its phosphorylation and nuclear translocation to exert its anti-bacterial function
作者：Wang, Ningning;Tan, Suxu;Wang, Muyuan;Liu, Hongning;Han, Sen;Wu, Zhendong;Ma, Jie;Chen, Songlin;Sha, Zhenxia;Wang, Ningning;Chen, Songlin;Chen, Songlin;Sha, Zhenxia
关键词：SHP-1; Immune response; Phosphorylation of NF kappa B1; Nuclear translocation
