Establishment of ultrasensitive immunoassay strip based on colloidal gold-McAb probe for detecting cyproheptadine hydrochloride and six phenothiazines in feed
文献类型： 外文期刊
作者： Hu, Xiaofei 1 ; Na, Guanqiong 1 ; Sun, Yaning 1 ; Xing, Yunrui 1 ; Chen, Linlin 3 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou, Peoples R China
2.Wenzhou Med Univ, Sch Publ Hlth & Management, Wenzhou, Peoples R China
3.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou, Peoples R China
4.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China
关键词： Monoclonal antibody; Cyproheptadine; Phenothiazines; Immunochromatographic strip; Rapid detection
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT （ 影响因子：2.9； 五年影响因子：3.2 ）
ISSN： 1944-0049
年卷期： 2023 年 40 卷 11 期
页码：
收录情况： SCI
摘要： An ultrasensitive and broad-specific monoclonal antibody recognising cyproheptadine hydrochloride and six phenothiazines was produced. The 50% inhibition concentration against cyproheptadine hydrochloride was 0.036 ng/mL, and the cross-reactivities for six phenothiazines were from 6.33% to 63.16%. Based on the developed monoclonal antibody, an immunochromatographic strip was established, with the visual detection limits (cut-off values) of seven drugs ranging from 5 to 100 ng/g in feedstuffs. With the strip reader, the 50% inhibition concentration of the developed immunochromatographic strip for seven drugs ranged from 0.570 to 7.750 ng/g. The intra-assay recoveries were from 79.8% to 103.4% with the highest coefficient of variation of 11.3%. The inter-assay recoveries were from 79.0% to 96.6% with the highest coefficient of variation of 12.7%. In summary, the proposed immunochromatographic strip was considered suitable for simultaneously monitoring cyproheptadine hydrochloride and phenothiazines in feedstuffs.
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