A transcriptome analysis of Benincasa hispida revealed the pathways and genes involved in response to Phytophthora melonis infection
文献类型： 外文期刊
作者： Cai, Jinsen 1 ; Yang, Songguang 1 ; Liu, Wenrui 1 ; Yan, Jinqiang 1 ; Jiang, Biao 1 ; Xie, Dasen 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China
关键词： RNA-Seq; Benincasa hispida; Phytophthora melonis; wilt disease; resistanceassociated gene
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Wilt disease caused by Phytophthora melonis infection is one of the most serious threats to Benincasa hispida production. However, the mechanism of the response of B. hispida to a P. melonis infection remains largely unknown. In the present study, two B. hispida cultivars with different degrees of resistance to P. melonis were identified: B488 (a moderately resistant cultivar) and B214 (a moderately susceptible cultivar). RNA-seq was performed on P. melonis-infected B488 and B214 12 hours post infection (hpi). Compared with the control, 680 and 988 DEGs were respectively detected in B488 and B214. A KEGG pathway analysis combined with a cluster analysis revealed that phenylpropanoid biosynthesis, plant-pathogen interaction, the MAPK signaling pathway-plant, and plant hormone signal transduction were the most relevant pathways during the response of both B488 and B214 to P. melonis infection, as well as the differentially expressed genes in the two cultivars. In addition, a cluster analysis of transcription factor genes in DEGs identified four genes upregulated in B488 but not in B214 at 6 hpi and 12 hpi, which was confirmed by qRT-PCR. These were candidate genes for elucidating the mechanism of the B. hispida response to P. melonis infection and laying the foundation for the improvement of B. hispida.
- 相关文献
作者其他论文 更多>>
-
Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)
作者：Wang, Min;Liu, Wenrui;Peng, Qingwu;Shi, Shaoqi;Jiang, Biao;Lin, Yu'e;Yang, Songguang;Wang, Min;Liu, Wenrui;Shi, Shaoqi;Cao, Liqin;Jiang, Biao;Zhao, Tianyue;Cui, Xiaojuan;Yang, Songguang;Wang, Ying
关键词：bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress
-
Time-course transcriptome analysis of the two types of seeds provides insights into seed shape differentiation in wax gourd
作者：Luo, Chen;Yan, Jinqiang;Wang, Min;Liu, Wenrui;Xie, Dasen;Jiang, Biao;Li, Zheng
关键词：Wax gourd; Seed shape; Transcriptome; Differentially expressed genes
-
Chromosome-level genome assembly and population genomics reveals crucial selection for subgynoecy development in chieh-qua
作者：Wang, Min;Cao, Zhenqiang;Jiang, Biao;Xie, Dasen;Chen, Lin;Shi, Shaoqi;Yang, Songguang;Peng, Qingwu;Wang, Kejian;Lu, Hongwei
关键词：
-
Identification of candidate genes controlling cucumber hypocotyl elongation under low light stress based on BSA-seq and RNA-seq
作者：Chen, Lin;Meng, Haoyuan;Chen, Baoying;Xie, Shuyan;Liu, Wenrui;Wang, Min;Yan, Jinqiang;Cai, Jinsen;Yang, Songguang;Jiang, Biao;Peng, Qingwu;Lin, Yu'e
关键词：Cucumber; Hypocotyl; Low light stress; BSA-seq; RNA-seq
-
Chemical compositions and cryo-adhesive probing of the epicuticular wax crystals on fruit surface of wax gourd (Benincasa hispida)
作者：Huang, Hua;Yan, Huaxue;Yan, Jinqiang;Jiang, Biao
关键词：Wax gourd; Epicuticular wax crystals; Cryo-adhesive; Scanning electronic microscope; Gas chromatography -mass spectrometry
-
A nonsynonymous mutation in BhLS, encoding an acyl-CoA N-acyltransferase leads to fruit and seed size variation in wax gourd (Benincasa hispida)
作者：Yan, Jinqiang;Xie, Dasen;Wang, Yi;Wang, Min;Yang, Songguang;Jiang, Biao;Chen, Lin;Cai, Jinsen;Liu, Wenrui;Cheng, Jiaowen
关键词：
-
Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd
作者：Liu, Wei;Wang, Min;Luo, Chen;Shi, Shaoqi;Qian, Yulei;Jiang, Biao;Liu, Wei;Zhong, Min;Kang, Yunyan;Wang, Min;Luo, Chen;Shi, Shaoqi;Qian, Yulei;Jiang, Biao
关键词：Wax gourd; Heat stress; Genome-wide identification; Evolutionary pattern; BhbZIP58
