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An ultrasensitive monoclonal antibody-based lateral flow immunoassay for the rapid detection of xylazine in milk

文献类型： 外文期刊

作者： Chen, Linlin 1 ; Hu, Xiaofei 2 ; Sun, Yaning 2 ; Xing, Yunrui 2 ; Zhang, Gaiping 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

关键词： Rapid detection; Xylazine; Monoclonal antibody; Immunoassay strip; Milk

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 383 卷

页码：

收录情况： SCI

摘要： In this study, a novel hapten was designed and successfully synthesized to prepared the artificial immunogen against xylazine (XYL), then the monoclonal antibody 1H2-2C6 was obtained and applied to a lateral flow immunoassay (LFI) strip for rapid detection of XYL in milk. The visual detection limit (cutoff value) of the LFI strip was 5 ng/mL, the linear regression formula was Y = -0.521lg(X) + 0.382 (R-2 = 0.995, n = 6), the linear detection range was 0.16-2.23 ng/mL and the detection limit (LOD) was 0.1 ng/mL. The recovery rates were in the range from 88.95% to 91.45%, the highest coefficient of variation was 8.89% (n = 3). The LFI strip was also validated by LC-MS/MS, as revealed, there were no significance differences between the two methods (the confidence interval was 95%). In brief, the LFI strip provides a reliable, sensitive and portable testing tool for screening of XYL on-site.

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