Expression of yeast Hem1 gene controlled by Arabidopsis HemA1 promoter improves salt tolerance in Arabidopsis plants
文献类型： 外文期刊
作者： Zhang, Zhi-Ping 1 ; Yao, Quan-Hong 1 ; Wang, Liang-Ju 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China
2.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China
关键词： Arabidopsis thaliana HemA1 promoter (AtHemA1 promoter);Saccharomyces cerevisiae Hem1 gene;Salt tolerance;Transgenic plant;5-Aminolevulinic acid (ALA)
期刊名称：BMB REPORTS （ 影响因子：4.778； 五年影响因子：4.377 ）
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收录情况： SCI
摘要： 5-Aminolevulinate (ALA) is well-known as an essential biosynthetic precursor of all tetrapyrrole compounds, which has been suggested to improve plant salt tolerance by exogenous application. In this work, the gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae Hem1) was introduced into the genome of Arabidopsis controlled by the Arabidopsis thaliana HernA1 gene promoter. All transgenic lines were able to transcribe the YHem1 gene, especially under light condition. The chimeric protein (YHem1-EGFP) was found co-localizing with the mitochondria in onion epidermal cells. The transgenic Arabidopsis plants could synthesize more endogenous ALA with higher levels of metabolites including chlorophyll and heme. When the T-2 homozygous seeds were cultured under NaCI stress, their germination and seedling growth were much better than the wild type. Therefore, introduction of ALA-S gene led to higher level of ALA metabolism with more salt tolerance in higher plants.
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