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Role of Alkaline Serine Protease, Asp, in Vibrio alginolyticus Virulence and Regulation of Its Expression by LuxO-LuxR Regulatory System

文献类型： 外文期刊

作者： Rui Haopeng 1 ; Liu, Qin 1 ; Wang, Qiyao 1 ; Ma, Yue 1 ; Liu, Huan 1 ; Shi, Cunbin 2 ; Zhang, Yuanxing 1 ;

作者机构： 1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

关键词： Alkaline serine protease;asp;quorum sensing;Vibrio alginolyticus

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

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收录情况： SCI

摘要： The alkaline serine protease asp, which was shown to be a virulence factor of vibrio alginolyticus as a purified protein, was cloned from V. alginolyticus EPGS, a strain recently isolated from moribund Epinephelus coioides in an outbreak of vibriosis in a mariculture farm of Shenzhen. The ash null mutant was constructed by homologous recombination with suicide plasmid pNQ705-1. Compared with the wild-type strain, the asp null mutant exhibited a significant decrease of total extracellular protease activity, and caused a 15-fold decrease in virulence of V. alginolyticus. In our previous study, the luxO and luxR(val) genes from V. alginolyticus MVP01 were cloned and identified, and the luxO-luxR(val) regulatory couple was shown to regulate various genes expression, suggesting that it played a central role in the quorum sensing system of V. alginolyticus. In this study, the regulation of the asp gene was analyzed by using RT-PCR and quantitative real-time PCR methods; we proved that its transcription was greatly induced at the late stage of growth and was regulated by a luxO-luxR(val) regulatory system.

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