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Isolation and characterization of polymorphic microsatellite DNA markers in the rock bream (Oplegnathus fasciatus)

文献类型: 外文期刊

作者: Xu, Tianjun 1 ; Shao, Changwei 1 ; Liao, Xiaolin 1 ; Ji, Xiangshan 1 ; Chen, Songlin 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China

2.Shanghai Fisheries Univ, Coll Aqua Life Sci & Technol, Shanghai 200090, Peoples R China

关键词: Oplegnathus fasciatus;Enriched genomic library;Microsatellites

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN:

年卷期:

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收录情况: SCI

摘要: From a (GT)(13)-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine, the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated from the Hardy-Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis and molecule-assisted breeding for O. fasciatus.

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