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Eighteen novel microsatellite markers for the Chinese sea perch, Lateolabrax maculatus

文献类型： 外文期刊

作者： Shao, Changwei 1 ; Chen, Songlin 1 ; Xu, Genbo 1 ; Liao, Xiaolin 1 ; Tian, Yongsheng 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

关键词： Chinese sea perch;Lateolabrax maculatus;Repeat-enriched genomic library;Microsatellites

期刊名称：CONSERVATION GENETICS （ 影响因子：2.538； 五年影响因子：2.32 ）

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收录情况： SCI

摘要： Chinese sea perch (Lateolabrax maculates) is one of the most important commercial species of mariculture in China. In this study, we constructed a repeat-enriched genomic DNA library of L. maculates. Eighteen dinucleotide microsatellite markers were characterized by genotyping 32 samples. The number of alleles ranged from three to nine, and the observed and expected heterozygosities ranged from 0.4516 to 1.0000 and from 0.4045 to 0.8676, respectively. Significant deviations from Hardy-Weinberg expectations were detected at four loci and linkage disequilibrium between two loci was significant after applying Bonferroni correction. The 18 highly polymorphic microsatellite markers should provide sufficient level of genetic diversity to investigate the population structure and evaluate the breeding strategy in L. maculates.

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