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Twelve novel polymorphic microsatellite loci for the Yellow grouper (Epinephelus awoara) and cross-species amplifications

文献类型: 外文期刊

作者: Zhao, Lili 1 ; Shao, Changwei 1 ; Liao, Xiaolin 1 ; Ma, Hongyu 1 ; Zhu, Xuechun 2 ; Chen, Songlin 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Nanchang Univ, Coll Life Sci, Nanchang 330031, Peoples R China

关键词: Yellow grouper;Epinephelus awoara;Enriched genomic library;Microsatellites

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN:

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收录情况: SCI

摘要: Yellow grouper (Epinephelus awoara) is a commercially important marine fish species. A dinucleotide-enriched genomic library of E. awoara was constructed using the method of FIASCO. Twelve loci were polymorphic in a test population with alleles per locus ranging from two to eight, and observed and expected heterozygosities per locus from 0.13 to 1.00 and from 0.20 to 0.86, respectively. Five loci significantly deviated from Hardy-Weinberg equilibrium and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species. These polymorphic microsatellite loci would be useful for investigating genetic diversity of E. awoara and related species.

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