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A sequence-selective electrochemical DNA biosensor based on HRP-labeled probe for colorectal cancer DNA detection

文献类型： 外文期刊

作者： Wang, Zhijie 1 ; Yang, Yunhui 2 ; Leng, Kailiang 1 ; Li, Jishan 2 ; Zheng, Fan 2 ; Shen, Guoli 2 ; Yu, Ruqin 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Cheometr, Changsha, Peoples R China

关键词： electrochemical DNA biosensor;HRP-labeled probe;colorectal cancer;self-assembly;sandwich

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： A highly-sensitive sequence-selective DNA sensor based on HRP-labeled probe to detect specific K-ras gene which is highly associated with colorectal cancer has been reported. Capture probe modified with-SH was first chemically adsorbed on the gold electrode through self-assembly. Then, the hybridization of a complementary nucleic acid (target DNA:K-ras gene) and HRP labeled oligonucleotide detection probe occurred in a sandwich way. Finally, H2O2 electroreduction current catalyzed by HRP was measured amperometrically in the presence of hydroquinon as mediator. The sequence selectivity is double guaranteed by the complementary hybridization of target DNA with capture and detection probes. The experimental conditions were optimized. The linear range is 1.17 x 10(-11) similar to 1.17 x 10(-7) mol 1(-1) with a detection limit of 5.85 x 10(-12) mol 1(-1). The electrode with capture probe can be reused after regeneration in boiling water.

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