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Characterization of myogenic regulatory factors, myod and myf5 from Megalobrama amblycephala and the effect of lipopolysaccharide on satellite cells in skeletal muscle

文献类型： 外文期刊

作者： Shi, Lin-Lin 1 ; Zhu, Ke-Cheng 2 ; Wang, Huan-Ling 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fishery, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Freshwater Anim Breeding,Minist Educ, Wuhan 430070, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China

关键词： Skeletal muscle; Myod; Myf5; Satellite cell; LPS; Megalobrama amblycephala

期刊名称：GENE （ 影响因子：3.913； 五年影响因子：3.48 ）

ISSN： 0378-1119

年卷期： 2022 年 834 卷

页码：

收录情况： SCI

摘要： Myod and Myf5 are muscle-specific basic helix-loop-helix (bHLH) transcription factors that play essential roles in regulating skeletal muscle development and growth. In order to investigate potential function of myod and myf5 of Megalobrama amblycephala, an economically important freshwater fish species, in the present study, we characterized the sequences and expression profiles of M. amblycephala myod and myf5. The open reading frame (ORF) sequences of myod and myf5 encoded 275 and 240 amino acids, respectively, possessing analogous structure with the highly conserved domains, bHLH and C-terminal helix III domains. Spatio-temporal expression patterns revealed that myod and myf5 were predominant in skeletal muscle with the highest expression in white muscle, and the highest at 10 days post-hatching (dph) and the segmentation period, respectively. Furthermore, we evaluated the effects of lipopolysaccharide (LPS) on the expression of muscle-related genes in white and red muscle, and proliferation and differentiation of satellite cells. The myod, myf5 and pax-7 expression generally increased and then decreased with increase of LPS concentration and treatment time in red muscle, while these genes showed inconsistent expression patterns in white muscle. In addition, LPS administration caused the frequency increase of satellite cells in red and white muscle especially at 3 and 7 days after LPS-injection.

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