Mito-Q promotes porcine oocytes maturation by maintaining mitochondrial thermogenesis via UCP2 downregulation
文献类型： 外文期刊
作者： Zhou, Dan 1 ; Zhuan, Qingrui 1 ; Luo, Yuwen 1 ; Liu, Hongyu 1 ; Meng, Lin 2 ; Du, Xingzhu 1 ; Wu, Guoquan 3 ; Hou, Yunpeng 2 ; Li, Jun 4 ; Fu, Xiangwei 1 ;
作者机构： 1.Coll Anim Sci, Key Lab Anim Genet, Natl Engn Lab Anim Breeding, Beijing Key Lab Anim Genet Improvement, Beijing 100193, Peoples R China
2.China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China
3.Yunnan Anim Sci & Vet Inst, Yunnan Prov Engn Lab Anim Genet Resource Conservat, Kunming 650224, Yunnan, Peoples R China
4.First Hosp Hebei Med Univ, Reprod Med Ctr, Dept Reprod Med, Shijiazhuang 050031, Hebei, Peoples R China
5.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi 832000, Xinjiang, Peoples R China
关键词： Oocyte; Mito-Q; UCP2; ATP; Thermogenesis
期刊名称：THERIOGENOLOGY （ 影响因子：2.923； 五年影响因子：2.843 ）
ISSN： 0093-691X
年卷期： 2022 年 187 卷
页码：
收录情况： SCI
摘要： Mitochondrial thermogenesis is an adaptive response of cells to their surrounding stress. Oxidative stress is one of the common stresses during in vitro maturation (IVM) of oocytes, which leads to mitochondrial dysfunction. This study aimed to probe the effects of the mitochondria-targeted antioxidant Mito-Q on oocyte development and unravel the role of Mito-Q in mitochondrial ATP production and thermogenesis regulation. Our results showed that Mito-Q had a positive effect on porcine oocytes maturation and subsequent embryo development. During oocytes IVM, Mito-Q could reduce ATP levels and ROS, increase lipid droplets accumulation, induce autophagy, and maintain mitochondrial temperature stability. Moreover, in metaphase II (MII) oocytes, Mito-Q would induce mitochondrial uncoupling manifested by decreased ATP, attenuated mitochondrial membrane potential (MMP), and increased mitochondrial thermogenesis. Notably, the expression of mitochondrial uncoupling protein (UCP2) was significantly reduced in oocytes treated with Mito-Q. Further study indicated that specific depletion of UCP2 in oocytes also resulted in increased thermogenesis, decreased ATP and declined MMP, suggesting that UCP2 downregulation may participate in Mito-Q-induced mitochondrial uncoupling. In summary, our data demonstrate that Mito-Q promotes oocyte maturation in vitro and maintains the stability of mitochondrial thermogenesis by inhibiting UCP2 expression.(c) 2022 Elsevier Inc. All rights reserved.
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