Development of Attenuated Total Reflectance Mid-Infrared (ATR-MIR) and Near-Infrared (NIR) Spectroscopy for the Determination of Resistant Starch Content in Wheat Grains
文献类型： 外文期刊
作者： Wang, Rong 1 ; Wei, Xia 1 ; Wang, Hongpan 1 ; Zhao, Linshu 5 ; Zeng, Cengli 6 ; Wang, Bingrui 7 ; Zhang, Wenying 1 ; Liu, 1 ;
作者机构： 1.Yangtze Univ, Hubei Key Lab Waterlogging Disaster & Agr Use Wet, Jingzhou, Hubei, Peoples R China
2.Yangtze Univ, Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Hubei, Peoples R China
3.Yangtze Univ, Minist Educ, Engn Res Ctr Ecol & Agr Use Wetland, Jingzhou 434025, Hubei, Peoples R China
4.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan 430064, Peoples R China
5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
6.Jianghan Univ, Hubei Engn Res Ctr Protect & Utilizat Special Bio, Wuhan 430056, Peoples R China
7.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430064, Peoples R China
期刊名称：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY （ 影响因子：2.193； 五年影响因子：2.4 ）
ISSN： 2090-8865
年卷期： 2021 年 2021 卷
页码：
收录情况： SCI
摘要： The chemical method for the determination of the resistant starch (RS) content in grains is time-consuming and labor intensive. Near-infrared (NIR) and attenuated total reflectance mid-infrared (ATR-MIR) spectroscopy are rapid and nondestructive analytical techniques for determining grain quality. This study was the first report to establish and compare these two spectroscopic techniques for determining the RS content in wheat grains. Calibration models with four preprocessing techniques based on the partial least squares (PLS) algorithm were built. In the NIR technique, the mean normalization + Savitzky-Golay smoothing (MN + SGS) preprocessing technique had a higher coefficient of determination (R-c(2) = 0.672; R-p(2) = 0.552) and a relative lower root mean square error value (RMSEC = 0.385; RMSEP = 0.459). In the ATR-MIR technique, the baseline preprocessing method exhibited a better performance regarding to the values of coefficient of determination (R-c(2) = 0.927; R-p(2) = 0.828) and mean square error value (RMSEC = 0.153; RMSEP = 0.284). The validation of the developed best NIR and ATR-MIR calibration models showed that the ATR-MIR best calibration model has a better RS prediction ability than the NIR best calibration model. Two high grain RS content wheat mutants were screened out by the ATR-MIR best calibration model from the wheat mutant library. There was no significant difference between the predicted values and chemical measured values in the two high RS content mutants. It proved that the ATR-MIR model can be a perfect substitute in RS measuring. All the results indicated that the ATR-MIR spectroscopy with improved screening efficiency can be used as a fast, rapid, and nondestructive method in high grain RS content wheat breeding.
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