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Real-time kinetics and affinity analysis of the interaction between protein A and immunoglobulins G derived from different species on silica colloidal crystal films

文献类型： 外文期刊

作者： Wang, Lu 1 ; Wan, Yizhen 1 ; Ma, Ning 1 ; Zhou, Lele 1 ; Zhao, Dongmin 2 ; Yu, Jianning 2 ; Wang, Huili 2 ; Lin, Zhiping 3 ; Qian, Weiping 1 ;

作者机构： 1.Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China

2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

3.Nanjing Weigang Dairy Co Ltd, Nanjing 211102, Peoples R China

关键词： Kinetics; Affinity; Silica colloidal crystal films; Protein A; Immunoglobulins G

期刊名称：COLLOIDS AND SURFACES B-BIOINTERFACES （ 影响因子：5.8； 五年影响因子：5.4 ）

ISSN： 0927-7765

年卷期： 2022 年 219 卷

页码：

收录情况： SCI

摘要： Kinetic and affinity analysis of protein interactions reveals information on their related activities in biological processes. Herein, we established a system for evaluating the kinetics and affinity of the interaction between protein A and various IgG species on the surface of silica spheres of silica colloidal crystal (SCC) films by the extraordinary optical interference capabilities of 190 nm silica spheres after self-assembly. The equilibrium association constant (KA) was calculated by the equilibrium Langmuir model and nonlinear least-squares analysis of time-dependent data. The relative protein A/IgG binding affinity is human > rabbit >cow >goat. In addition, the competitive interaction of distinct species of IgG with protein A at the interface of SCC films was studied and performed. These findings may help with the use of protein A and other recognition components in a number of sensor types. Furthermore, this research might offer a novel approach to determining the kinetics and affinity of proteins on the surface of spheres particles, which may contribute to the development of the application of spheres particles in pharmaceutical science, biomedical engineering, and other techniques.

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