文献类型： 外文期刊

作者： Meng, Meng ¹ ; Ning, Fang ¹ ; He, Xiaoyang ¹ ; Li, Huihui ¹ ; Feng, Yinyin ¹ ; Qi, Yanlong ² ; Sun, Huiqing ¹ ;

作者机构： 1.Tianjin Univ Sci & Technol, Coll Food Sci & Engn, State Key Lab Food Nutr & Safety, Minist Educ, 29,13th Ave,Tianjin Econ Technol Dev Area, Tianjin 300457, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Agr Prod Proc, Xinjiang Key Lab Proc & Preservat Agr Prod, 403 Nanchang Rd, Urumqi 830091, Peoples R China

关键词： blackened jujube; polysaccharide; structural characterization; immunoregulatory activity

期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）

ISSN：

年卷期： 2025 年 14 卷 14 期

页码：

收录情况： SCI

摘要： Previously, research adopted an ultrasound-assisted extraction method to isolate crude polysaccharide from blackened jujube, followed by preliminary structural identification of the purified polysaccharide (BJP). This manuscript analyzed the accurate structure and immunomodulatory activity of BJP. Further structural identification indicated that BJP was mainly composed of -> 3)-alpha-L-Araf-(1 ->, -> 3,5)-alpha-L-Araf-(1 ->, -> 3)-beta-D-GalpA-(1 ->, -> 2,4)-beta-D-Galp-(1 ->, -> 4)-beta-D-GalpA-(1 ->, -> 3)-alpha-L-Rhap-(1 -> and -> 3,4)-alpha-L-Rhap-(1 ->. The immunomodulatory effects of BJP were examined using a mouse model with immunosuppression induced by cyclophosphamide. The findings suggested that BJP could relieve the condition of immunosuppressed mice. BJP could inhibit decreases in the body weight and organ index of mice, and HE staining showed that BJP could alleviate the harm to spleen and thymus tissues. BJP enhanced the secretion of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2), interleukin-6 (IL-6), immunoglobulin A (IgA), and immunoglobulin G (IgG) in serum. It also reduced liver oxidative stress by increasing superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) activities, while lowering malondialdehyde (MDA) levels. Moreover, BJP contributed to the maintenance of gut homeostasis by stimulating the generation of short-chain fatty acids in the cecal contents. The study aims to establish a solid basis for the comprehensive development of blackened jujube and furnish a theoretical framework for its polysaccharides' role in immune modulation.