文献类型： 外文期刊

作者： Meng, Meng ¹ ; Sun, Ying ¹ ; Qi, Yanlong ² ; Xu, Jin ¹ ; Sun, Jingge ¹ ; Bai, Yuhe ¹ ; Han, Lirong ³ ; Han, Ran ¹ ; Hou, Lihua ¹ ; Sun, Huiqing ¹ ;

作者机构： 1.Tianjin Univ Sci Technol, State Key Lab Food Nutr & Safety, Key Lab Food Nutr & Safety, Minist Educ,Coll Food Sci Engn, 29,13th Ave, Tianjin 300457, Peoples R China

2.Xinjiang Acad Agr Sci, Synthet Test Site, 1 Xiaokang Rd, Urumqi 830000, Peoples R China

3.Hebei Univ, Minist Educ, Key Lab Publ Hlth Safety Hebei Prov, Baoding 071002, Peoples R China

4.Hebei Univ, Coll Publ Hlth, Baoding 071002, Peoples R China

关键词： Purple sweet potato; Polysaccharide; Structural analysis; Antitumor; Apoptosis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 235 卷

页码：

收录情况： SCI

摘要： The carbohydrate is the main ingredient of purple sweet potato. A polysaccharide, named PSP, was separated and purified from purple sweet potato by extraction with hot water, precipitation with ethanol, deproteinization with Sevag reagent and column chromatography with Sephadex G-100. The purity and structure were studied with HPLC, UV-Vis, GC-MS and NMR. The PSP is a neutral polysaccharide with Mw of 470 kDa. The monosaccharide composition of PSP contained D-xylose, D-glucose, D-galactose with ratio of 1.0: 8.3: 1.3. The backbone of PSP was composed of the residues of -> 6)-D-Glcp-(1 -> and -> 2, 6)-D-Glcp-(1 ->. The branches of PSP contained the residues of -> 3)-D-Galp-(1 ->, and D-Xylp-(1 ->. The antitumor activity in vitro of PSP was analyzed with HT-29 cells. And the SEM, AO staining, MDC staining and hoechst 33342 staining were performed to study the effect on apoptosis of HT-29 cells by PSP. The results revealed that the PSP can significantly inhibit the proliferation of HT-29 cells from induction apoptosis. The manuscript provided valuable knowledges on structural characteristics of the polysaccharides from purple sweet potato.