iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A
文献类型： 外文期刊
作者： Liu, Lu 1 ; Hao, Liaoyang 3 ; Liu, Ning 4 ; Zhao, Yonglong 3 ; Zhong, Naiqin 2 ; Zhao, Pan 2 ;
作者机构： 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
3.Ningxia Univ, Sch Agr, Yinchuan 750021, Ningxia, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
5.Chinese Acad Sci, Engn Lab Adv Microbial Technol Agr, Beijing 100101, Peoples R China
6.Enterprise Key Lab Adv Technol Potato Fertilizer, Hulunbuir 021000, Peoples R China
关键词： Solanum tuberosum; common scab; thaxtomin A; proteomic analysis; iTRAQ
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.924； 五年影响因子：6.132 ）
ISSN：
年卷期： 2021 年 22 卷 21 期
页码：
收录情况： SCI
摘要： Thaxtomin A (TA) is a phytotoxin secreted by Streptomyces scabies that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation-reduction process, response to stress, plant-pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.
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