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Global proteomic analysis of serum during early pregnancy in the pig using data-independent acquisition mass spectrometry with verification by parallel reaction monitoring

文献类型： 外文期刊

作者： Xin, Haiyun 1 ; Meng, Fanming 1 ; Li, Baohong 1 ; Wang, Sutian 1 ; Hu, Bin 1 ; Li, Jianhao 1 ;

作者机构： 1.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding, Guangdong Key Lab Anim Breeding & Nutr, Inst Anim Sci, Guangzhou 510640, Peoples R China

2.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming 525000, Guangdong, Peoples R China

3.Qingyuan Longfa Pig Breeding Co Ltd, Guangdong Engn Technol Res Ctr Pig Breeding, Yingde 513057, Guangdong, Peoples R China

关键词： differentially expressed proteins; pig; pregnancy diagnosis; quantitative proteomics; reproduction; serum; Tibetan swine

期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：1.973； 五年影响因子：2.124 ）

ISSN： 1031-3613

年卷期：

页码：

收录情况： SCI

摘要： Context. The current pregnancy diagnosis is generally not ideal in accuracy and efficiency, and the physiological process of early pregnancy in pig remains unclarified. Aims. This study aimed to evaluate protein expression profiles and identify typical proteins of early pregnancy for more understanding of physiological processes. Methods. Data-independent acquisition-based (DIA) quantitative proteomic analysis was performed to compare the serum proteome profiles on days 0, 5, 12, 16, and 19 of gestation in Tibetan pig. Parallel reaction monitoring (PRM) was subsequently performed to verify relative expression level. Key results, 396 proteins were detected, of which 113 differentially expressed proteins (DEPs) were identified. Functional annotation and pathway analysis indicated that the DEPs were mainly involved in catalytic activity, metabolic processes and the proteasome. Four candidate DEPs (talin 1, profilin, carbonic anhydrase, and HGF activator) showed consistent expression trends in both DIA and PRM approaches. Conclusions. The DIA based proteomic methods indicate the involvement of numerous serum proteins in early pregnancy physiological function in pigs. The combination of DIA-PRM based global proteomic analysis may provide insights for function study and pregnancy diagnosis biomarkers. Implications. The global proteomic analyses performed here have increased the knowledge of early pregnancy in Tibetan swine and provide potential methods for pregnancy detection.

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