您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Enhanced phytoremediation of selenium using genetically engineered rice plants

文献类型： 外文期刊

作者： Li, Zhenjun 1 ; Tian, Yongsheng 1 ; Wang, Bo 1 ; Peng, Rihe 1 ; Xu, Jing 1 ; Fu, Xiaoyan 1 ; Han, Hongjuan 1 ; Wang, Lijuan 1 ; Zhang, Wenhui 1 ; Deng, Yongdong 1 ; Wang, Yu 1 ; Gong, Zehao 1 ; Gao, Jianjie 1 ; Yao, Quanhong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotechnol Res Inst, 2901 Beidi Rd, Shanghai 201106, Peoples R China

关键词： Phytoremediation; Rice; Selenium; Selenocysteine lyase; Selenocysteine methyltransferase

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.686； 五年影响因子：4.352 ）

ISSN： 0176-1617

年卷期： 2022 年 271 卷

页码：

收录情况： SCI

摘要： Selenium (Se) is a micronutrient essential for human and animal health. However, Se is toxic at high levels because the nonspecific substitution of cysteine by selenocysteine could lead to protein malfunction. In an attempt to prevent nonspecific selenocysteine incorporation into proteins, we simultaneously overexpressed the gene encoding selenocysteine lyase from Homo sapiens (HsSL), which specifically catalyzes the decomposition of selenocysteine into elemental Se-0 and alanine, and the gene encoding selenocysteine methyltransferase from Astragalus bisulcatus (AbSMT), which methylates selenocysteine into methylselenocysteine in rice. The transgenic plants showed normal growth under standard conditions. Se treatment resulted in higher levels of alanine and methylselenocysteine in transgenic plants than in wild-type plants, which indicated that this approach might have successfully redirected Se flow in the plant. Overexpression of HsSL and AbSMT in rice also endows transgenic plants with hyposensitivity to Se stress at the seed germination stage. The transgenic plants showed enhanced selenate and selenite tolerance, which was simultaneously supported by fresh weight values. Moreover, our phytoremediation assay revealed that the transgenic plants exhibited greatly improved Se elimination capabilities and accumulated about 38.5% and 128.6% more Se than wild-type plants when treated with selenate and selenite, respectively. This study offers hope that genetically modified plants could play a role in the restoration of Se-contaminated environment.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台