Integrated transcriptomic and metabolomic analyses reveal key genes controlling flavonoid biosynthesis in Citrus grandis 'Tomentosa' fruits
文献类型： 外文期刊
作者： Fan, Ruiyi 1 ; Zhu, Congyi 1 ; Qiu, Diyang 1 ; Mao, Genlin 1 ; Mueller-Roeber, Bernd 2 ; Zeng, Jiwu 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Minist Agr & Rural Affairs,Guangdong Prov Key Lab, Guangzhou 510640, Peoples R China
2.Univ Potsdam, Inst Biochem & Biol, D-14476 Potsdam, Germany
3.Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany
4.Ctr Plant Syst Biol & Biotechnol CPSBB, 139 Ruski Blvd, Plovdiv 4000, Bulgaria
关键词： Citrus grandis ?Tomentosa?; Transcriptome; Flavonoids; Traditional Chinese medicine
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）
ISSN： 0981-9428
年卷期： 2023 年 196 卷
页码：
收录情况： SCI
摘要： As a well-recognized traditional Chinese medicine (TCM), immature fruits of Citrus grandis 'Tomentosa' (CGT) serve to cure chronic cough in humans. Specialized metabolites including flavonoids may have contribute to this curing effect. Knowledge about the molecular mechanisms underlying flavonoid biosynthesis in 'Tomentosa' fruits will, therefore, support the breeding of varieties with improved medicinal properties. Hence, we profiled the transcriptomes and metabolites of the fruits of two contrasting C. grandis varieties, namely 'Zheng-Mao' ('ZM') used in TCM production, and a locally cultivated pomelo, namely 'Guang-Qing' ('GQ'), at four devel-opmental stages. A total of 39 flavonoids, including 14 flavanone/flavone, 5 isoflavonoids, 12 flavonols, and 6 anthocyanins, were identified, and 16 of which were quantitatively determined in the fruits of the two varieties. We found that 'ZM' fruits contain more flavonoids than 'GQ'. Specifically, rhoifolin levels were significantly higher in 'ZM' than in 'GQ'. We annotated 31,510 genes, including 1,387 previously unknown ones, via tran-scriptome sequencing of 'ZM' and 'GQ.' A total of 646 genes were found to be differentially expressed between 'ZM' and 'GQ' throughout at all four fruit developmental stages, indicating that they are robust expression markers for future breeding programs. Weighted gene co-expression network analysis identified 18 modules. Combined transcriptional and metabolic analysis revealed 25 genes related to flavonoid biosynthesis and 16 transcriptional regulators (MYBs, bHLHs, WD40) that may be involved in the flavonoids biosynthesis in C. grandis 'Tomentosa' fruits.
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