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Establishment, characterization and application in germplasm conservation and disease resistance: An embryonic cell line from Yangtze sturgeon (Acipenser dabryanus)

文献类型： 外文期刊

作者： Gong, Zhihong 1 ; Zhang, Qi 2 ; Liu, Juanjuan 4 ; Hu, Guobin 1 ; Chen, Songlin 2 ; Wang, Na 2 ;

作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Laoshan Lab, Qingdao 266071, Peoples R China

3.Zhejiang Ocean Univ, Fisheries Coll, Zhoushan 316022, Peoples R China

4.China Three Gorges Corp, Chinese Sturgeon Res Inst, Yichang 443100, Hubei, Peoples R China

5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China

关键词： Acipenser dabryanus; Embryo; Cell line; Differentiation; Immunology

期刊名称：AQUACULTURE （ 2022影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2023 年 575 卷

页码：

收录情况： SCI

摘要： Yangtze sturgeon (Acipenser dabryanus), is a particularly valuable and endangered fish species distributed in the Yangtze River of China. Bacterial diseases outbreaks have caused catastrophic losses in sturgeon farming. To conserve Yangtze sturgeon germplasm resources and facilitate the research of sturgeon etiology without harming individual, the first A. dabryanus embryonic cell line-ADEc was successfully established from its neural plate embryo at 72 h after fertilization. ADEc cells were cultured in Leibovitz's L-15 (L-15) medium containing 20% fetal bovine serum at 25 degrees C and were stably passaged up to 50 generations. Karyotyping analysis has indicated that the chromosome number for most ADEc cells was 2n = 234. The successful transfection of Cy3-labeled scramble siRNA and GFP reporter gene into ADEc cells were observed. The alkaline phosphatase (AP) staining showed that there were few cells showing pluripotency. The differentiation of ADEc cells toward to neuronal cell type could be observed after all-trans retinoic acid (RA) induction. In addition, embryonic stem cell markerwdr43 and neural stem cell markers such as bmi1, nes, and ocln were expressed in ADEc cells. Transcriptome sequencing revealed that 1562 differentially expressed genes were identified at 2 h after lipopolysaccharide (LPS) stimulation, which significantly enriched into the Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, and NF-kappa B signaling pathway. The establishment of ADEc cell line will help to conserve germplasm resources and provide useful in vitro research tools for immunological, biological, and genomic studies of Yangtze sturgeon.

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