Manipulated C5aR1 over/down-expression associates with IL-6 expression during bacterial inflammation in half-smooth tongue sole (Cynoglossus semilaevis)
文献类型： 外文期刊
作者： Wu, Zhendong 1 ; Zang, Shaoqing 1 ; Wang, Wenwen 1 ; Tan, Suxu 1 ; Xu, Qian 1 ; Chen, Xuejie 4 ; Han, Sen 1 ; Ma, Jie 1 ; Shi, Kunpeng 1 ; Wang, Ningning 1 ; Cheng, Jiayu 5 ; Sha, Zhenxia 1 ;
作者机构： 1.Qingdao Univ, Inst Aquat Biotechnol, Coll Life Sci, Qingdao 266071, Peoples R China
2.Qingdao Univ, Coll Basic Med, Qingdao 266071, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
5.Tangshan Weizhuo Aquaculture Co Ltd, Engn & Technol Ctr Flatfish Aquaculture Tangshan, Tangshan 063202, Peoples R China
关键词： C5aR1; IL-6; Cynoglossus semilaevis; Bacterial inflammation; Vibrio anguillarum; Mycobacterium marinum
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）
ISSN： 1050-4648
年卷期： 2024 年 151 卷
页码：
收录情况： SCI
摘要： The complement component 5a/complement component 5 receptor 1 (C5a/C5aR1) pathway plays a crucial role in the onset and development of inflammation, but relevant studies in fish are lacking. In this study, we successfully characterized the relationship between half-smooth tongue sole (Cynoglossus semilaevis) C5aR1 (CsC5aR1) and bacterial inflammation. First, we showed that the overexpression of CsC5aR1 significantly increased bacterial pathological damage in the liver and intestine, whereas inhibition attenuated the damage. The in vitro experiments suggested that CsC5aR1 was able to positively regulate the phagocytic activity and respiratory burst of tongue sole macrophages. In terms of both transcriptional and translational levels, overexpression/inhibition of CsC5aR1 was followed by a highly consistent up-regulation/decrease of its downstream canonical inflammatory factor interleukin-6 (CsIL-6). Furthermore, we stimulated macrophages by lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and found a broad-spectrum response to bacterial infections by the C5a/C5aR1 complement pathway together with the downstream inflammatory factor CsIL-6. Subsequently, we directly elucidated that CsIL-6 is an indicator of C5a/C5aR1-mediated inflammation at different infection concentrations, different infectious bacteria (Vibrio anguillarum and Mycobacterium marinum), and different detection levels. These results might provide a new inflammation bio-marker for early warning of bacteria-induced hyperinflammation leading to fish mortality and a promising target for the treatment of bacterial inflammation in teleost.
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