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Biodegradation of p-nitrophenol by engineered strain

文献类型： 外文期刊

作者： Xu, Jing 1 ; Wang, Bo 1 ; Zhang, Wen-hui 1 ; Zhang, Fu-Jian 1 ; Deng, Yong-dong 1 ; Wang, Yu 1 ; Gao, Jian-Jie 1 ; Tian, 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

关键词： p-Nitrophenol; Degradation; Multigene metabolic engineering; E; coli; Bioremediation

期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）

ISSN： 2191-0855

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： SCI

摘要： p-Nitrophenol (PNP) is an important environmental pollutant and can causes significant environmental and health risks. Compared with the traditional methods, biodegradation is a useful one to completely remove the harmful pollutants from the environment. Here, an engineered strain was first constructed by introducing PNP biodegradation pathway via the hydroquinone (HQ) pathway into Escherichia coli. In the engineered strain BL-PNP, PNP was completely degraded to beta-ketoadipate and subsequently enter the metabolites of multiple anabolic pathways. The high tolerance and rapid degradation ability to PNP enable the engineered strain to have the potential to degrade toxic substances. The engineered strain created in this study can be used as a functional strain for bioremediation of PNP and potential toxic intermediates, and the method of assembling aromatic hydrocarbons metabolic pathway can be used to eradicate nitroaromatic pollutants in the environment.

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