Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase
文献类型： 外文期刊
作者： Zou, Gen 1 ; Bao, Dapeng 1 ; Wang, Ying 1 ; Zhou, Sichi 1 ; Xiao, Meili 2 ; Yang, Zhanshan 1 ; Wang, Yinmei 2 ; Zhou, Zhi 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai Key Lab Agr Genet & Breeding, 1000 Jinqi Rd, Fengxian 201403, Shanghai, Peoples R China
2.Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Synthet Biol, Fenglin Rd 300, Shanghai 200032, Peoples R China
关键词： Product inhibition; Cellulase; Lignocellulose; Trichoderma reesei
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2021 年 319 卷
页码：
收录情况： SCI
摘要： Product inhibition of cellulase is a challenging issue in industrial processes. Here, we introduced a product activated mushroom cellulase, PaCel3A from Polyporus arcularius, into Trichoderma reesei. The filter paper activity, carboxymethyl cellulase activity, and saccharification efficiency (substrate: pretreated rice straw, PRS) of transformants increased significantly with this enzyme (by 18.4-26.8%, 13.8-22.8%, and 17.0%, respectively). A mutant of PaCel3A, PaCel3AM, obtained based on B-factor analysis, saturated mutagenesis, and residual activity assay, showed improved thermostability. The PRS saccharification efficiency using the cellulase complex from T. reesei transformants overexpressing pacel3am increased by 56.4%-63.0%. In addition, the T. reesei cellulase complex obtained by adding the purified recombinant PaCel3AM from T. reesei (rCel3aM-tr) to hydrolyze PRS resulted in increased reducing sugar yields at all sampling points, outperforming the cellulase complexes without rCel3aM-tr. These results suggest that introducing product-activated cellulase genes is a simple and feasible method to alleviate the product inhibition of cellulase.
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