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Role of aspartate ammonia-lyase in Pasteurella multocida

文献类型： 外文期刊

作者： Wang, Zui 1 ; Li, Li 1 ; Liu, Peng 1 ; Wang, Chen 1 ; Lu, Qin 1 ; Liu, Lina 1 ; Wang, Xiaozhong 2 ; Luo, Qingping 1 ; Shao, 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Special One, Wuhan 430064, Peoples R China

2.Anim Dis Prevent & Control Ctr Yichang, Yichang 443000, Peoples R China

3.Key Lab Prevent & Control Agents Anim Bacteriosis, Special 1, Wuhan 430064, Peoples R China

关键词： Pasteurella multocida; Aspartate ammonia-lyase; Iron acquisition; Virulence

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Pasteurella multocida is responsible for a highly infectious and contagious disease in birds, leading to heavy economic losses in the chicken industry. However, the pathogenesis of this disease is poorly understood. We recently identified an aspartate ammonia-lyase (aspA) in P. multocida that was significantly upregulated under iron-restricted conditions, the protein of which could effectively protect chicken flocks against P. multocida. However, the functions of this gene remain unclear. In the present study, we constructed aspA mutant strain oaspA::kan and complementary strain CoaspA::kan to investigate the function of aspA in detail. Result Deletion of the aspA gene in P. multocida resulted in a significant reduction in bacterial growth in LB (Luria-Bertani) and MH (Mueller-Hinton) media, which was rescued by supplementation with 20 mM fumarate. The mutant strain oaspA::kan showed significantly growth defects in anaerobic conditions and acid medium, compared with the wild-type strain. Moreover, growth of oaspA::kan was more seriously impaired than that of the wild-type strain under iron-restricted conditions, and this growth recovered after supplementation with iron ions. AspA transcription was negatively regulated by iron conditions, as demonstrated by quantitative reverse transcription-polymerase chain reaction. Although competitive index assay showed the wild-type strain outcompetes the aspA mutant strain and oaspA::kan was significantly more efficient at producing biofilms than the wild-type strain, there was no significant difference in virulence between the mutant and the wild-type strains. Conclusion These results demonstrate that aspA is required for bacterial growth in complex medium, and under anaerobic, acid, and iron-limited conditions.

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