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Identification of Myomaker in Yellowfin Seabream (Acanthopagrus latus) (Hottuyn, 1782) and its Transcriptional Regulation by Two MyoDs

文献类型： 外文期刊

作者： Zhu, Kecheng 1 ; He, Peiying 1 ; Liu, Baosuo 1 ; Guo, Huayang 1 ; Zhang, Nan 1 ; Guo, Liang 1 ; Jiang, Shigui 1 ; Zhang, 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

3.Guangdong Prov Engineer Technol Res Ctr Marine Bi, Guangzhou, Guangdong, Peoples R China

4.Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou, Guangdong, Peoples R China

关键词： Acanthopagrus latus; Myomaker; MyoD; Promoter activity; Mutation analyses

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2021 年 53 卷 1 期

页码：

收录情况： SCI

摘要： Myomaker is a muscle-specific membrane protein that is essential for myoblast fusion. Myomaker is regulated by myoblast determination protein (MyoD), a muscle-specific basic helix-loop-helix (bHLH) transcription factor in higher vertebrates. However, the transcriptional regulatory mechanism of the myomaker gene has not been explored in marine fishes. In the present study, molecular cloning, bioinformatic analysis and transcriptional analysis of Acanthopagrus latus myomaker (Altnyomaker) were performed. The open reading frame (ORF) sequence of Almyomaker is 858 bp, which encodes a polypeptide of 285 amino acids. Moreover, phylogenetic and gene structure analysis indicates that Almyomaker is highly conserved among vertebrates. The tissue distribution pattern shows that Alinyomaker is more highly expressed in white muscle than in other tissues. Furthermore, to explore whether two MyoDs are modulators of Almyomaker, a promoter analysis was performed using progressive deletion mutations of Almyomaker. The results of promoter activity assays show that Almyomaker expression is notably activated by two MyoDs. Transcriptional activity of the Almyomaker promoter was observed to dramatically decrease after targeted mutation of the MyoD1 M1 and MyoD2 M2 binding sites. In summary, MyoD1 and MyoD2 play an important role in the regulation ofAlmyomaker expression and may promote myoblast fusion during muscle development and growth by modulating Almyomaker expression.

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