Cloning and functional analysis of c/ebp alpha as negative regulator of dmrt1 in Chinese tongue sole (Cynoglossus semilaevis)
文献类型： 外文期刊
作者： Wang, Qian 1 ; Wang, Rui 1 ; Feng, Bo 1 ; Li, Shuo 1 ; Mahboob, Shahid 5 ; Shao, Changwei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
5.King Saud Univ, Coll Sci, Dept Zool, Riyadh 11451, Saudi Arabia
关键词： c/ebp alpha; Transcription factor; Testis; dmrt1; Gonadal differentiation; Cynoglossus semilaevis
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN： 0378-1119
年卷期： 2021 年 768 卷
页码：
收录情况： SCI
摘要： c/ebp alpha is a member of the C/EBP family of transcription factors, which are involved in cell growth and differentiation and have a conserved basic leucine zipper (bZIP) domain. However, little is known about its function in sex determination and differentiation. In the present study, c/ebp alpha was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of c/ebp alpha was 1583 bp, with a 198-bp 5' UTR, a 446-bp 3' UTR, and a 939-bp open reading frame encoding a 312-amino acid peptide. qRT-PCR revealed that c/ebp alpha was predominantly expressed in undifferentiated gonads of male C. semilaevis at 30 dpf and 60 dpf and peaked at 60 dpf. Expression levels of c/ebp alpha in the testis were constantly higher than those in ovaries at all developmental stages. Moreover, a dual-luciferase assay revealed that c/ebp alpha could negatively regulate the male-determining gene dmrt1 in vitro. These results provide fundamental information indicating that C. semilaevis c/ebp alpha might be involved in early gonadal differentiation and functions as a negative regulator of dmrt1 by repressing its transcription.
- 相关文献
作者其他论文 更多>>
-
Flow Velocity Modulates Growth, Oxidative Stress, and Transcriptomic Responses in Spotted Sea Bass (Lateolabrax maculatus)
作者：Li, Shuo;Wang, Qian;Liu, Baoliang;Shao, Changwei;Li, Shuo;Liu, Yuyan;Wang, Qian;Zhu, Zhiwen;Li, Weijing;Li, Chen;Fei, Fan;Liu, Baoliang;Shao, Changwei;Liu, Yuyan;Wang, Qian;Shao, Changwei
关键词：Spotted sea bass; Flow velocity; Oxidative stress; Lipid metabolism; Transcriptomics
-
Near complete genome assembly of Yadong trout (Salmo trutta)
作者：Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Li, Chen;Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Shao, Changwei
关键词：
-
Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning
作者：Pang, Yue;Du, Zeyu;Zhang, Jin;Lu, Jiali;Li, Jun;Dong, Xinrui;Zhao, Zhisheng;Chuan, Shunqin;Sun, Mingjie;Li, Qingwei;Pang, Yue;Du, Zeyu;Zhang, Jin;Lu, Jiali;Li, Jun;Dong, Xinrui;Zhao, Zhisheng;Chuan, Shunqin;Sun, Mingjie;Li, Qingwei;Qin, Yating;Liu, Qun;Han, Kai;Yuan, Zengbao;Pan, Shanshan;Xu, Mengyang;Wang, Dantong;Li, Zhen;Chen, Yadong;Song, Yue;Zhan, Liping;Cui, Wei;Wang, Jun;Fan, Guangyi;Qin, Yating;Liu, Qun;Han, Kai;Fan, Guangyi;Qin, Yating;Liu, Qun;Han, Kai;Song, Yue;Qin, Yating;Fan, Guangyi;Yuan, Zengbao;Xu, Mengyang;Wang, Dantong;Gu, Ying;Yang, Huanming;Xu, Xun;Liu, Xin;Fan, Guangyi;Xu, Mengyang;Fan, Guangyi;Li, Shuo;Zhang, Zhe;Ni, Ming;Jia, Xiaodong;Xia, Zhangyong;Yue, Zhen;Fan, Guangyi;Gu, Ying;Yang, Huanming;Xu, Xun;Liu, Xin
关键词：
-
Engineering a 3D platform for testis bioengineering: generation and proteomic profiling of decellularized fish testicular scaffolds
作者：Rosa, Ivana Felipe;Souza, Beatriz Marques;Nobrega, Rafael Henrique;Rosa, Ivana Felipe;Doretto, Lucas Benites;Rodrigues, Maira da Silva;Shao, Changwei;Rosa, Ivana Felipe;Doretto, Lucas Benites;Shao, Changwei;Barquilha, Caroline Nascimento;Fioretto, Matheus Naia;Portela, Luiz Marcos Frediani;Justulin, Luis Antonio;Padilha, Pedro de Magalhaes
关键词：testicular scaffolds; decellularization; sds; matrix extracellular; Astyanax lacustris
-
Establishment and identification of spleen cell line from tiger puffer fish (Takifugu rubripes) and immune transcriptome response to IHNV
作者：He, Yangbin;Zhang, Jingjing;Meng, Bo;He, Yangbin;Zhang, Jingjing;Lin, Lei;Meng, Bo;Dong, Caichao;Liu, Yuyan;Yang, Yu;Ji, Honglong;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei
关键词：Tiger puffer fish; Spleen; Cell line; Immunology; Transcriptome
-
High-quality chromosome-level genome assembly of the whitespotted conger (Conger myriaster)
作者：Han, Shenglei;Han, Shenglei;Li, Chen;Li, Shuo;Chen, Yan;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shi, Bao;Shao, Changwei;Han, Shenglei;Li, Chen;Li, Shuo;Chen, Yan;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shi, Bao;Shao, Changwei;Wang, Chenggang;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shao, Changwei
关键词：
-
Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum)
作者：Liu, Yi;Yang, Yexin;Wang, Yuanyuan;Liu, Chao;Ouyang, Guochang;Xu, Meng;Mu, Xidong;Bian, Chao;Shi, Qiong;Ma, Ka Yan;Ouyang, Guochang;Sun, Jinhui;Shao, Changwei;Chen, Jiehu;Yang, Yexin
关键词：Silver arowana; Whole-genome sequencing and assembly; Chromosome interaction; Transcriptome; Female-specific region;
foxl2
