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Cloning and functional analysis of c/ebp alpha as negative regulator of dmrt1 in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Wang, Qian 1 ; Wang, Rui 1 ; Feng, Bo 1 ; Li, Shuo 1 ; Mahboob, Shahid 5 ; Shao, Changwei 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

4.Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

5.King Saud Univ, Coll Sci, Dept Zool, Riyadh 11451, Saudi Arabia

关键词： c/ebp alpha; Transcription factor; Testis; dmrt1; Gonadal differentiation; Cynoglossus semilaevis

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2021 年 768 卷

页码：

收录情况： SCI

摘要： c/ebp alpha is a member of the C/EBP family of transcription factors, which are involved in cell growth and differentiation and have a conserved basic leucine zipper (bZIP) domain. However, little is known about its function in sex determination and differentiation. In the present study, c/ebp alpha was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of c/ebp alpha was 1583 bp, with a 198-bp 5' UTR, a 446-bp 3' UTR, and a 939-bp open reading frame encoding a 312-amino acid peptide. qRT-PCR revealed that c/ebp alpha was predominantly expressed in undifferentiated gonads of male C. semilaevis at 30 dpf and 60 dpf and peaked at 60 dpf. Expression levels of c/ebp alpha in the testis were constantly higher than those in ovaries at all developmental stages. Moreover, a dual-luciferase assay revealed that c/ebp alpha could negatively regulate the male-determining gene dmrt1 in vitro. These results provide fundamental information indicating that C. semilaevis c/ebp alpha might be involved in early gonadal differentiation and functions as a negative regulator of dmrt1 by repressing its transcription.

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