Transcriptome Analysis of Porcine Granulosa Cells in Healthy and Atretic Follicles: Role of Steroidogenesis and Oxidative Stress
文献类型： 外文期刊
作者： Meng, Li 1 ; Wu, Zhenfang 1 ; Zhao, Kun 1 ; Tao, Jian 1 ; Chit, Tam 3 ; Zhang, Shouquan 1 ; Wang, Chi Chiu 3 ; Teerds, Ka 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Li Ka Shing Inst Hlth Sci, Dept Obstet & Gynaecol,Shatin, Hong Kong, Peoples R China
4.Wageningen Univ, Human & Anim Physiol, Elst 1, NL-6708 WD Wageningen, Netherlands
关键词： transcriptome profiles; steroidogenesis; oxidative stress; antral follicular atresia
期刊名称：ANTIOXIDANTS （ 影响因子：6.312； 五年影响因子：6.648 ）
ISSN：
年卷期： 2021 年 10 卷 1 期
页码：
收录情况： SCI
摘要： One of the main causes of female infertility is a deregulated antral follicular atresia, a process of which the underlying molecular mechanisms are largely unknown. Our objective was therefore to characterize the complex transcriptome changes in porcine granulosa cells of healthy antral (HA) and advanced antral atretic (AA) follicles, using ELISA and RNA-Seq followed by qRT-PCR and immunohistochemistry. Granulosa cell RNA-Seq data revealed 2160 differentially expressed genes, 1483 with higher and 677 with lower mRNA concentrations in AA follicles. Bioinformatic analysis showed that the upregulated genes in AA follicles were highly enriched in inflammation and apoptosis processes, while the downregulated transcripts were mainly highlighted in the steroid biosynthesis pathway and response to oxidative stress processes including antioxidant genes (e.g., GSTA1, GCLC, GCLM, IDH1, GPX8) involved in the glutathione metabolism pathway and other redox-related genes (e.g., RRM2B, NDUFS4). These observations were confirmed by RT-qPCR and immunohistochemistry. Additionally, the granulosa cells of AA follicles express significantly stronger 8-OHdG immunostaining, a marker of oxidative DNA damage, implicating that oxidative stress may participate in follicular atresia. We hypothesize that the decrease in anti-apoptotic factors and steroid hormones coincides with increased oxidative stress markers and the expression of pro-apoptotic factors, all contributing to antral follicular atresia.
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