Development of a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for the detection of KHV
文献类型： 外文期刊
作者： Li, Yingying 1 ; Wang, Qing 1 ; Hu, Feng 1 ; Wang, Yingying 1 ; Bergmann, Sven M. 3 ; Zeng, Weiwei 4 ; Yin, Jiyuan 1 ; Sh 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol Guangdong Prov, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
3.Fed Res Inst Anim Hlth, Friedrich Loffler Inst FLI, Inst Infectol, German Reference Lab KHVD, Greifswald, Germany
4.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan, Peoples R China
关键词： double‐ antibody sandwich ELISA (DAS‐ ELISA); Koi herpesvirus (KHV); ORF132; polyclonal antibody
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期：
页码：
收录情况： SCI
摘要： Koi herpesvirus disease (KHVD) caused by the koi herpesvirus (KHV) is difficult to diagnose in live fish, presenting a challenge to the koi industry. The enzyme-linked immunosorbent assay (ELISA) method cannot be widely used to detect KHV because few commercial anti-KHV antibody exists. Here, we developed an anti-ORF132 polyclonal antibody and confirmed its reactivity via indirect immunofluorescence assay and Western blotting. A double-antibody sandwich ELISA (DAS-ELISA) was established to detect KHV, monoclonal antibody 1B71B4 against ORF92 was used as the capture antibody, and the detection antibody was the polyclonal antibody against the truncated ORF132. The lowest limit was 1.56 ng/ml KHV. Furthermore, the DAS-ELISA reacted with KHV isolates, while no cross-reactions occurred with carp oedema virus, spring viraemia of carp virus, frog virus 3 and grass carp reovirus. Two hundred koi serum samples from Guangdong, China, were used in the DAS-ELISA test, and the positive rate of the koi sera was 13%. The clinical sensitivity and specificity of the DAS-ELISA relative to the traditional PCR method were 66.7% and 97.6%, respectively. Our findings may be useful for diagnosing and preventing KHVD in koi and common carp.
- 相关文献
作者其他论文 更多>>
-
Identification and characterization of Streptococcus iniae from farmed American Bullfrogs (Aquarana catesbeiana)
作者：Sun, Jingyang;Li, Hua;Lin, Han;Chen, Kesong;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Huang, Yanhua;Liu, Chun;Wang, Qing;Wang, Qing;Huang, Yanhua;Liu, Chun
关键词：Identification; Characterization; Streptococcus iniae; American Bullfrog
-
Diet with optimal glutathione supplement improves growth, nonspecific immunity, intestinal microbiota, and antioxidant ability in Micropterus salmoides
作者：Xiong, Chuanyu;Chi, Yuyu;Li, Yun;Ye, Hua;Yin, Jiyuan;Wu, Ronghua;Xiong, Chuanyu;Chi, Yuyu;Li, Yun;Ye, Hua;Wu, Ronghua;Xiong, Chuanyu;Chi, Yuyu;Li, Yun;Ye, Hua;Wu, Ronghua;Wang, Bei;Yi, Jianhua;Yu, Yongyao
关键词：antioxidant capacity; growth parameters; intestinal microbiota; Micropterus salmoides; nonspecific immunity; reduced glutathione
-
Effects of ocean acidification and polystyrene microplastics on the oysters Crassostrea gigas: An integrated biomarker and metabolomic approach
作者：Du, Yunchao;Teng, Jia;Zhao, Jianmin;Ren, Jingying;Zhang, Tianyu;Wang, Qing;Du, Yunchao;Teng, Jia;Zhao, Jianmin;Ren, Jingying;Zhang, Tianyu;Wang, Qing;Du, Yunchao;Teng, Jia;Zhao, Jianmin;Ren, Jingying;Zhang, Tianyu;Wang, Qing;Sun, Shan;Xia, Bin;Ma, Hengyuan
关键词：Ocean acidification; Microplastics; Crassostrea gigas; Metabolomics; Combined effects; Biomarker; Multiple stresses; Climate change
-
Occurrence of herpesvirus in fish
作者：Bergmann, Sven Michael;Klafack, Sandro;Jin, Yeonhwa;Hofmann, Arndt Christian;Zeng, Weiwei;Bergmann, Sven Michael;Wang, Yingying;Li, Yingying;Wang, Qing;Zeng, Weiwei;Kielpinska, Jolanta;Becker, Anna Maria
关键词：herpesviruses; fish; clinical signs
-
Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection
作者：Wu, Huiliang;Yin, Jiyuan;Jiang, Peng;Wang, Linchuan;Wang, Qing;Li, Siming;Yan, Han;Wang, Hao;Ding, Zhaoyang;Li, Pengfei;Chen, Bo;Wang, Qing;Wang, Qing
关键词：Grass carp reovirus; VP4; Oral immunization; L. lactis; Immunogenicity; Protective efficacy
-
Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus x female Channa maculata ) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)
作者：Deng, Huiling;Zheng, Shucheng;Li, Yingying;Mo, Xubing;Zhao, Jian;Yin, Jiyuan;Shi, Cunbin;Wang, Qing;Wang, Yingying;Deng, Huiling;Zheng, Shucheng;Wang, Qing;Wang, Yingying
关键词：CAMK cell line; Hybrid snakehead; Virology; Hybrid snakehead rhabdovirus
-
Interferon regulatory factors inhibit TiLV replication by activating interferon-a3 in tilapia (Oreochromis niloticus)
作者：Ke, Zishan;Wen, Jing;Wang, Yingying;Li, Bo;Zhang, Defeng;Mo, Xubing;Li, Yingying;Ren, Yan;Yin, Jiyuan;Shi, Cunbin;Wang, Qing;Zheng, Shucheng;Ke, Zishan;Li, Bo;Zheng, Shucheng;Wu, Siyu;Zheng, Shucheng
关键词：Interferon regulatory factor; Interferon; Innate immunity; Tilapia; Tilapia lake virus
