文献类型： 外文期刊

作者： Guo, Hong-Wei ¹ ; Chang, Juan ¹ ; Wang, Ping ¹ ; Yin, Qing-Qiang ¹ ; Liu, Chao-Qi ¹ ; Xu, Xiao-Xiang ¹ ; Dang, Xiao-We ¹ ;

作者机构： 1.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China

2.Henan Delin Biol Product Co Ltd, Xinxiang 453000, Henan, Peoples R China

3.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

4.Henan Guangan Biotechnol Co Ltd, Zhengzhou 450001, Peoples R China

关键词： Aflatoxin B-1; Compound probiotics; Mycotoxin-degradation enzyme; Chicken embryo primary cells; Cell damage alleviation

期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）

ISSN： 2191-0855

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Aflatoxin B-1 (AFB(1)) is one of the most dangerous mycotoxins for humans and animals. This study aimed to investigate the effects of compound probiotics (CP), CP supernatant (CPS), AFB(1)-degradation enzyme (ADE) on chicken embryo primary intestinal epithelium, liver and kidney cell viabilities, and to determine the functions of CP+ADE (CPADE) or CPS+ADE (CPSADE) for alleviating cytotoxicity induced by AFB(1). The results showed that AFB(1) decreased cell viabilities in dose-dependent and time-dependent manners. The optimal AFB(1) concentrations and reactive time for establishing cell damage models were 200 mu g/L AFB(1) and 12 h for intestinal epithelium cells, 40 mu g/L and 12 h for liver and kidney cells. Cell viabilities reached 231.58% (p<0.05) for intestinal epithelium cells with CP addition, 105.29% and 115.84% (p<0.05) for kidney and liver cells with CPS additions. The further results showed that intestinal epithelium, liver and kidney cell viabilities were significantly decreased to 87.12%, 88.7% and 84.19% (p<0.05) when the cells were exposed to AFB(1); however, they were increased to 93.49% by CPADE addition, 102.33% and 94.71% by CPSADE additions (p<0.05). The relative mRNA abundances of IL-6, IL-8, TNF-alpha, iNOS, NF-kappa B, NOD1 (except liver cell) and TLR2 in three kinds of primary cells were significantly down-regulated by CPADE or CPSADE addition, compared with single AFB(1) group (p<0.05), indicating that CPADE or CPSADE addition could alleviate cell cytotoxicity and inflammation induced by AFB(1) exposure through suppressing the activations of NF-kappa B, iNOS, NOD1 and TLR2 pathways.