The ATR-WEE1 kinase module inhibits the MAC complex to regulate replication stress response
文献类型： 外文期刊
作者： Wang, Lili 1 ; Zhan, Li 1 ; Zhao, Yan 1 ; Huang, Yongchi 1 ; Wu, Chong 1 ; Pan, Ting 1 ; Qin, Qi 1 ; Xu, Yiren 1 ; Deng, Zhi 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Zhejiang, Peoples R China
期刊名称：NUCLEIC ACIDS RESEARCH （ 影响因子：16.971； 五年影响因子：15.542 ）
ISSN： 0305-1048
年卷期： 2021 年 49 卷 3 期
页码：
收录情况： SCI
摘要： DNA damage response is a fundamental mechanism to maintain genome stability. The ATR-WEE1 kinase module plays a central role in response to replication stress. Although the ATR-WEE1 pathway has been well studied in yeasts and animals, how ATR-WEE1 functions in plants remains unclear. Through a genetic screen for suppressors of the Arabidopsis atr mutant, we found that loss of function of PRL1, a core subunit of the evolutionarily conserved MAC complex involved in alternative splicing, suppresses the hypersensitivity of atr and weel to replication stress. Biochemical studies revealed that WEE1 directly interacts with and phosphorylates PRL1 at Serine 145, which promotes PRL1 ubiquitination and subsequent degradation. In line with the genetic and biochemical data, replication stress induces intron retention of cell cycle genes including CYCD1;1 and CYCD3;1, which is abolished in weel but restored in weel pil1. Remarkably, co-expressing the coding sequences of CYCD1;1 and CYCD3;1 partially restores the root length and HU response in weel prl1. These data suggested that the ATR-WEE1 module inhibits the MAC complex to regulate replication stress responses. Our study discovered PRL1 or the MAC complex as a key downstream regulator of the ATR-WEE1 module and revealed a novel cell cycle control mechanism.
- 相关文献
作者其他论文 更多>>
-
The tRNA thiolation-mediated translational control is essential for plant immunity
作者：Zheng, Xueao;Chen, Hanchen;Wu, Yujing;Wu, Chong;Yu, Xiaodan;Yan, Shunping;Zheng, Xueao;Chen, Qiansi;Zheng, Xueao;Chen, Hanchen;Wu, Yujing;Wu, Chong;Yu, Xiaodan;Yan, Shunping;Zheng, Xueao;Chen, Hanchen;Wu, Yujing;Wu, Chong;Yu, Xiaodan;Yan, Shunping;Zheng, Xueao;Chen, Hanchen;Wu, Yujing;Wu, Chong;Yu, Xiaodan;Yan, Shunping;Zheng, Xueao;Chen, Hanchen;Wu, Yujing;Wu, Chong;Yu, Xiaodan;Yan, Shunping;Deng, Zhiping;Zhong, Linlin
关键词：plant immunity; translation; tRNA thiolation; NPR1; Arabidopsis; A. thaliana
-
The m6A reader SlYTH2 negatively regulates tomato fruit aroma by impeding the translation process
作者：Bian, Hanxiao;Gao, Ying;Wang, Hanqing;Ye, Bingbing;Chen, Kunsong;Klee, Harry J.;Zhang, Bo;Song, Peizhe;Cai, Zhihe;Jia, Guifang;Gao, Ying;Deng, Zhiping;Huang, Chenyang;Liu, Jianzhao;Yu, Lei;Gao, Xiangwei;Pan, Yu;Wang, Fengqin;Jia, Guifang;Jia, Guifang;Zhang, Bo
关键词：phase separation; translation repression; RNA methylation; flavor quality
-
The kiwifruit amyloplast proteome (kfALP): a resource to better understand the mechanisms underlying amyloplast biogenesis and differentiation
作者：Li, Ang;Lin, Jiajia;Zeng, Zhebin;Chen, Xiaoya;Ding, Gang;Zhu, Man;Xu, Bin;Cheng, Yunjiang;Deng, Xiuxin;Zeng, Yunliu;Deng, Zhiping;Tan, Jinjuan;Atkinson, Ross G.;Nieuwenhuizen, Niels J.;Ampomah-Dwamena, Charles
关键词：Actinidia; amyloplast biogenesis; amyloplast differentiation; plastids; development; fruit quality; ripening
-
EGR1 and EGR2 positively regulate plant ABA signaling by modulating the phosphorylation of SnRK2.2
作者：Li, Chuanling;Li, Xuetong;Song, Yuning;Liu, Xinye;Li, Ziye;Zhang, Ya;Zhang, Baowen;Tang, Wenqiang;Shang, Jian-Xiu;Sun, Yu;Li, Chuanling;Deng, Zhiping;Tang, Xiaohan Alex
关键词：abscisic acid; Arabidopsis thaliana; EGR1; EGR2; protein phosphorylation; serine 31; SnRK2.2
-
Global-scale no-tillage impacts on soil aggregates and associated carbon and nitrogen concentrations in croplands: A meta-analysis
作者：Li, Peng;Wu, Choufei;Li, Peng;Deng, Jianjun;Li, Chunkai;Jiao, Jiaguo;Hu, Feng;Ying, Duo;Li, Jing;Tian, Shanyi;Zhao, Gen;Jiang, Min
关键词：No-tillage; Straw retention; Aggregates; Soil carbon and nitrogen concentration; Meta-analysis
-
A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain
作者：Yuan, Zhengjie;Geng, Yanfei;Dai, Yuanxing;Li, Jing;Lv, Mingfang;Zhang, Heng-Mu;Dai, Yuanxing;Liao, Qiansheng;Xie, Li
关键词：cell death; fijivirus; transmembrane domain; viral nonstructural protein
-
Integrated transcriptome and metabolome analyses shed light on the defense mechanisms in tomato plants after (E)-2-hexenal fumigation
作者：Zhang, Jihong;Liang, Quanwu;Li, Yuqiong;Song, Ge;Wang, Haihua;Yan, Mingli;Deng, Zhiping;Wang, Xuewen
关键词：Tomato ( Solanum lycopersicum ); (E)-2-hexenal; Metabolomic; Transcriptomic; Amino acid biosynthesis
