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Global Transcriptomic Analyses Reveal Genes Involved in Conceptus Development During the Implantation Stages in Pigs

文献类型： 外文期刊

作者： Zang, Xupeng 1 ; Gu, Ting 1 ; Hu, Qun 1 ; Xu, Zhiqian 1 ; Xie, Yanshe 1 ; Zhou, Chen 1 ; Zheng, Enqin 1 ; Huang, Sixiu 1 ; X 1 ;

作者机构： 1.South China Agr Univ, Natl Engn Res Ctr Breeding Swine Ind, Coll Anim Sci, Guangzhou, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Coll Anim Sci, Guangzhou, Peoples R China

3.Lingnan Guangdong Lab Modern Agr, Guangzhou, Peoples R China

4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Peoples R China

关键词： pig; conceptus; implantation; development; RNA-Seq

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Prenatal mortality remains a significant concern to the pig farming industry around the world. Spontaneous fetal loss ranging from 20 to 45% by term occur after fertilization, with most of the loss happening during the implantation period. Since the factors regulating the high mortality rates of early conceptus during implantation phases are poorly understood, we sought to analyze the overall gene expression changes during this period, and identify the molecular mechanisms involved in conceptus development. This work employed Illumina's next-generation sequencing (RNA-Seq) and quantitative real-time PCR to analyze differentially expressed genes (DEGs). Soft clustering was subsequently used for the cluster analysis of gene expression. We identified 8236 DEGs in porcine conceptus at day 9, 12, and 15 of pregnancy. Annotation analysis of these genes revealed rRNA processing (GO:0006364), cell adhesion (GO:1904874), and heart development (GO:0007507), as the most significantly enriched biological processes at day 9, 12, and 15 of pregnancy, respectively. In addition, we found various genes, such as T-complex 1, RuvB-like AAA ATPase 2, connective tissue growth factor, integrins, interferon gamma, SLA-1, chemokine ligand 9, PAG-2, transforming growth factor beta receptor 1, and Annexin A2, that play essential roles in conceptus morphological development and implantation in pigs. Furthermore, we investigated the function of PAG-2 in vitro and found that PAG-2 can inhibit trophoblast cell proliferation and migration. Our analysis provides a valuable resource for understanding the mechanisms of conceptus development and implantation in pigs.

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