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FucP mediates fucose to regulate T3SS in Edwardsiella piscicida and promotes intestinal colonization in Tilapia

文献类型： 外文期刊

作者： Ye, Tingqi 1 ; Wu, Jiayan 3 ; Mu, Cuimin 1 ; Pan, Guangchen 1 ; Chen, Jiakang 1 ; Wang, Xuepeng 1 ;

作者机构： 1.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

2.Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An 271018, Shandong, Peoples R China

3.Chinese Acad Fishery Sci, Qingdao Natl Lab Marine Sci & Technol, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 272000, Peoples R China

4.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China

关键词： Edwardsiella piscicida; Fucose; FucP; T3SS; Colonization

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：1.363； 五年影响因子：1.75 ）

ISSN： 0967-6120

年卷期： 2021 年 29 卷 3 期

页码：

收录情况： SCI

摘要： Edwardsiella piscicida (E. piscicida) is a common pathogen in freshwater and marine environments and an important intestinal pathogen of fish. Fucose large presence in the intestines could possibly regulate relevant genes to promote bacterial infectivity. In this study, the wild-type E. piscicida and the fucP gene-deleted strain Delta fucP were used to identify its virulence-regulating pathway and colonization. The results showed that the gene transcription levels of key FusKR and type III secretion system (T3SS) in E. piscicida were upregulated by different concentrations of fucose. Both the wild-type and the Delta fucP strain grew faster with 30 mM fucose added than that without fucose added. In addition, the penetration of the wild-type E. piscicida with fucose added was significantly stronger than that of both Delta fucP with fucose and the wild-type E. piscicida without fucose. Meanwhile, the gene transcription levels of T3SS-related genes eseB, eseC, eseD, eseE, and eseG in E. piscicida were significantly downregulated after the fucP gene mutant. Finally, tilapia infected with the wild-type strain showed a significant increase in the E. piscicida colonization compared to those infected with the mutant strain. All results indicate that FucP of E. piscicida mediated fucose to promote the growth, motility, and intestinal colonization in tilapia.

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