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The role of uhpA in Edwardsiella piscicida and the inflammatory cytokine response in tilapia

文献类型： 外文期刊

作者： Sun, Yongcan 1 ; Chen, Jiakang 1 ; Liu, Jinyu 1 ; Huang, Jinjing 1 ; Ye, Tingqi 1 ; Wang, Xuepeng 1 ;

作者机构： 1.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

2.Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An 271018, Shandong, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao 272000, Peoples R China

关键词： Edwardsiella piscicida; UhpA; Pathogenicity; Cytokines; Tilapia

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 101 卷

页码：

收录情况： SCI

摘要： Edwardsiella piscicida (E. piscicida) is an important zoonotic pathogen that infects fish by colonizing the intestines. The intestine provides nutrition including Glucose 6-phosphate (Glu6P) and a competitive environment for the microbiota. Although the transport system regulatory protein gene uhpA has been reported in E. piscicida genomes, whether the uhpA gene is involved in the pathogenicity of E. piscicida remains largely unknown. Therefore, the uhpA gene mutants strain E. piscicida Delta uhpA was constructed to elucidate the functions of Glu6P and the uhpA gene in E. piscicida. The results demonstrated that Glu6P significantly increased the gene expression of uhpC/uhpB/uhpA than without adding Glu6P in the culture. The gene expression of uhpC and uhpB was down regulated in the mutant strain than that of in the wild type strain. E. piscicida Delta uhpA exhibited an increase in virulence compared to that of E. piscicida EIB202 [LD50 value: (3.98 x 10(6) CFU/fish) and LD50 value: (1.45 x 10(7) CFU/fish) respectively]. Besides, although TNF-alpha did not show significant differences (p > 0.05) in the spleen of tilapia infected with Delta uhpA and EIB202 in the whole observed period, the gene expression of IL-15 and TGE-beta in the spleen of tilapia infected with Delta uhpA showed significantly higher (p < 0.05) than that of in tilapia infected with EIB202. Meanwhile, the gene expression of IL-1 beta and TGE-beta in spleen of tilapia infected with Delta uhpA showed significantly higher (p < 0.05) than that of in fish infected with EIB202 when zebrafish used as the control in the whole observed period. All these results suggested that Glu6P up-regulated the gene expression of uhpC/uhpB/uhpA; most important, the uhpA gene deletion in E. piscicida down-regulated the gene expression of uhpC and uhpB, enhanced its pathogenicity and its role in inducing the inflammatory cytokine responses in tilapia.

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