您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

文献类型： 外文期刊

作者： Wang, Qishuo 1 ; Zhang, Chao 4 ; Xu, La 5 ; Chen, Jiakang 1 ; Wang, Xuepeng 1 ;

作者机构： 1.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China

2.Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An, Shandong, Peoples R China

3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

4.Chongqing Three Gorges Vocat Coll, Chongqing, Peoples R China

5.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Maricuiture, Qingdao, Peoples R China

6.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China

关键词： bacterial ghosts; challenge; immune response; Streptococcus iniae; tilapia

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2021 年 52 卷 4 期

页码：

收录情况： SCI

摘要： Streptococcus iniae (S. iniae) is a Gram-positive bacterium associated with disease in several commercial species and other animals including humans. This zoonotic pathogen has led to high worldwide morbidity and mortality and huge economic losses in aquaculture. Here a S. iniae ghost (SIG) vaccine was successfully generated with a chemical method, and its immunogenicity and protective efficacy were examined in this study. The results showed that the titres of immunoglobulin M (IgM) in serum were significantly higher in group A (SIG-immunized) than group B (formalin-inactivated S. iniae, FSI-immunized), which was significantly higher than the control group (PBS-treated). Also, the phagocytic activity was significantly higher in group A than group B, which was significantly higher than the control group. Moreover, AKP activity and lysozyme activity of serum in group A were significantly higher than group B, which was significantly higher than the control group. Also, IL-1 beta, IFN-gamma and HSP70 in the head kidney of fish immunized with SIG and FSI showed significantly higher than the control group throughout the whole experimental period. In addition, IL-1 beta and IFN-gamma in the spleen of fish immunized with SIG showed significantly higher than that of in the FSI-immunized fish. Most important, the survival rate and relative per cent survival of group A were higher than group B, and both of them were all significantly more protected against the S. iniae challenge compared to the PBS-treated group. These results suggest that immunization with SIG induces immune responses and provides protection against a virulent S. iniae challenge.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台