African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2
文献类型： 外文期刊
作者： Chen, Sheng 1 ; Zhang, Xinheng 1 ; Nie, Yu 1 ; Li, Hongxin 1 ; Chen, Weiguo 1 ; Lin, Wencheng 1 ; Chen, Feng 1 ; Xie, Qin 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Peoples R China
2.Lingnan Guangdong Lab Modern Agr, Guangzhou 510642, Peoples R China
3.Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China
4.South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Peoples R China
5.Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Peoples R China
关键词： African swine fever virus (ASFV); E199L; Pyrroline-5-carboxylate reductase 2 (PYCR2); Autophagy
期刊名称：VIROLOGICA SINICA （ 影响因子：3.242； ）
ISSN： 1674-0769
年卷期： 2021 年 36 卷 2 期
页码：
收录情况： SCI
摘要： African swine fever virus (ASFV), as a member of the large DNA viruses, may regulate autophagy and apoptosis by inhibiting programmed cell death. However, the function of ASFV proteins has not been fully elucidated, especially the role of autophagy in ASFV infection. One of three Pyrroline-5-carboxylate reductases (PYCR), is primarily involved in conversion of glutamate to proline. Previous studies have shown that depletion of PYCR2 was related to the induction of autophagy. In the present study, we found for the first time that ASFV E199L protein induced a complete autophagy process in Vero and HEK-293T cells. Through co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) analysis, we firstly identified that E199L interact with PYCR2 in vitro. Importantly, our work provides evidence that E199L down-regulated the expression of PYCR2, resulting in autophagy activation. Overall, our results demonstrate that ASFV E199L protein induces complete autophagy through interaction with PYCR2 and down-regulate the expression level of PYCR2, which provide a valuable reference for the role of autophagy during ASFV infection and contribute to the functional clues of PYCR2.
- 相关文献
作者其他论文 更多>>
-
Analysis of metagenome and metabolome disclosed the mechanisms of Dendrobium officinale polysaccharide on DSS-induced ulcerative colitis-affected mice
作者：Zeng, Xiaona;Tang, Shengqiu;Dong, Xiaoying;Zeng, Xiaona;Dong, Mengyue;Shao, Runlin;Liu, Ruiheng;Li, Tong;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Dong, Mengyue;Shao, Runlin;Liu, Ruiheng;Li, Tong;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Tang, Shengqiu;Dong, Xiaoying;Zeng, Xiaona;Dong, Mengyue;Shao, Runlin;Liu, Ruiheng;Li, Tong;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Dong, Mengyue;Shao, Runlin;Liu, Ruiheng;Li, Tong;Zhang, Xinheng;Xie, Qingmei;Wong, Yung Hou;Wong, Yung Hou
关键词：Dendrobium officinale polysaccharide; Ulcerative colitis (UC); Metagenome; Metabolome
-
Exosomal circ_CCDC7/gga-miR-6568-3p/Pax7 axis accelerates the differentiation of chicken embryonic stem cells infected with subgroup J avian leukosis virus
作者：Zeng, Xiaona;Wang, Ruonan;Chen, Sheng;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Wang, Ruonan;Liao, Liqin;Chen, Sheng;Kong, Jie;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Tang, Shengqiu;Dong, Xiaoying;Xie, Qingmei;Zeng, Xiaona;Wang, Ruonan;Liao, Liqin;Chen, Sheng;Kong, Jie;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Wang, Ruonan;Liao, Liqin;Chen, Sheng;Kong, Jie;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Tang, Shengqiu;Li, Yajuan;Wong, Yung Hou;Wong, Yung Hou
关键词：circRNA; ALV-J; chicken embryonic stem cell
-
Resveratrol Enhances Antioxidant and Anti-Apoptotic Capacities in Chicken Primordial Germ Cells through m6A Methylation: A Preliminary Investigation
作者：Qiao, Yanzhao;Xiao, Gengsheng;Zhang, Xinheng;Kong, Jie;Xie, Qingmei;Qiao, Yanzhao;Xiao, Gengsheng;Zhang, Xinheng;Kong, Jie;Xie, Qingmei;Qiao, Yanzhao;Xiao, Gengsheng;Bu, Yonghui;Zhang, Xinheng;Kong, Jie;Xie, Qingmei;Qiao, Yanzhao;Xiao, Gengsheng;Zhang, Xinheng;Kong, Jie;Xie, Qingmei;Zhu, Xiaohua;Wen, Jun;Bai, Yinshan;Zhu, Xiaohua;Wen, Jun;Bai, Yinshan
关键词：primordial germ cells; resveratrol; antioxidant; apoptosis; m6A methylation
-
Small RNAs contribute to citrus Huanglongbing tolerance by manipulating methyl salicylate signaling and exogenous methyl salicylate primes citrus groves from emerging infection
作者：Cheng, Baoping;Peng, Aitian;Cheng, Baoping;Peng, Aitian;Xu, Le;Bilal, Muhammad Saqib;Huang, Qing;Niu, Dongdong;Ma, Hongyu;Zhou, Shaoxia;Zhao, Hongwei;Xu, Le;Bilal, Muhammad Saqib;Huang, Qing;Niu, Dongdong;Ma, Hongyu;Zhou, Shaoxia;Zhao, Hongwei;Wei, Guo;Chen, Feng;Zeng, Liang;Lin, Hong;Baig, Ayesha;Wang, Xuefeng;Zou, Xiuping
关键词：microRNA; immunity; methyl salicylate; community immunity; gene silencing; Shatangju
-
Critical role for ribonucleoside-diphosphate reductase subunit M2 in ALV-J-induced activation of Wnt/β-catenin signaling via interaction with P27
作者：Tang, Shuang;Leng, Mei;Tan, Chen;Zhu, Lin;Pang, Yanling;Zhang, Xinheng;Lin, Wencheng;Chang, Yung-Fu
关键词：ALV-J; RRM2; Wnt/beta-catenin signaling; replication
-
Molecular characterization, complete genome sequencing, and pathogenicity of Novel Duck Reovirus from South Coastal Area in China
作者：Kong, Jie;Shao, Guanming;Zhang, Yukun;Wang, Jinfeng;Xie, Zi;Feng, Keyu;Zhang, Xinheng;Xie, Qingmei;Kong, Jie;Shao, Guanming;Zhang, Yukun;Wang, Jinfeng;Xie, Zi;Feng, Keyu;Zhang, Xinheng;Xie, Qingmei;Kong, Jie;Shao, Guanming;Zhang, Yukun;Wang, Jinfeng;Xie, Zi;Feng, Keyu;Zhang, Xinheng;Xie, Qingmei;Shao, Guanming;Feng, Keyu;Feng, Keyu;Kong, Jie;Shao, Guanming;Zhang, Yukun;Wang, Jinfeng;Xie, Zi;Feng, Keyu;Zhang, Xinheng;Xie, Qingmei
关键词：Novel Duck Reovirus; isolated; complete genome; phylogenetic analysis; pathogenicity
-
Analysis of long noncoding RNAs and messenger RNAs expression profiles in the hearts of mice with acute viral myocarditis
作者：Xue, Yimin;Chen, Mingguang;Zeng, Lijuan;Fan, Qiaolian;Ke, Jun;Zhang, Jiuyun;Chen, Feng;Ke, Jun;Zhang, Jiuyun;Chen, Feng;Zheng, Chunfu;Zheng, Chunfu;Chen, Feng
关键词：acute viral myocarditis; coxsackievirus B3; long noncoding RNAs; messenger RNAs; RNA sequencing
