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Antiviral activity of porcine interferon delta 8 against pesudorabies virus in vitro

文献类型： 外文期刊

作者： Zhang, Teng 1 ; Liu, Yunchao 1 ; Chen, Yumei 2 ; Wang, Jucai 1 ; Feng, Hua 1 ; Wei, Qiang 1 ; Zhao, Shuangshuang 1 ; Yang 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China

2.Zhengzhou Univ, Sch Life Sci, Zhengzhou, Peoples R China

3.Northwest A&F Univ, Coll Vet Med, Yangling, Shaanxi, Peoples R China

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China

5.Henan Zhongze Biol Engn Co Ltd, Zhengzhou, Peoples R China

6.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： Pseudorabies virus; A stably cell line; IFN-88; Antiviral activity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：5.162； 五年影响因子：5.137 ）

ISSN： 0141-8130

年卷期： 2021 年 177 卷

页码：

收录情况： SCI

摘要： Recently, pseudorabies virus (PRV) was isolated from human cases, and infected patients presented with respiratory dysfunction and acute neurological symptoms. However, there was no available effective drug to prevent the progression of PRV infection. In the present study, we screened a stably Drosophila S2 cell line which can secretory express a novel type I IFNs-interferon delta 8 (IFN-88) and the yield was about 10 mg/L. After purifica-tion, recombinant IFN-88 was demonstrated to be acid-stable, heat-stable, and nontoxic to PK-15 and 3D4/21 cells. Antiviral effects of IFN-88 against PRV were tested in vitro. Our results showed both pre-and posttreatment, recombinant PoIFN-88 exerted a significant protective effect against PRV infection in PK-15 and 3D4/21 cells. In addition, PoIFN-88 remarkably increased the expression of eight IFN-stimulated genes (ISGs), including ISG15, OAS1, PKR, MX1, CH25H, IFITM1, IFITM2 and IFITM3, to resist virus infection. These findings highlight the significance of IFN-88 that might serve as an antiviral agent for the prevention of PRV infection, and maybe expand the potential function of IFN antiviral drugs in the future. ? 2021 Elsevier B.V. All rights reserved.

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