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Evaluation of CVC1302 for Improved Efficacy of FMD-Inactivated Vaccine in Oxidative Stressed Mice Generated with PCV2 Infection

文献类型： 外文期刊

作者： Hou, Liting 1 ; Zhang, Chenxin 1 ; Yu, Xiaoming 1 ; Zhang, Yuanpeng 1 ; Du, Luping 1 ; Chen, Jin 1 ; Zheng, Qisheng 1 ; H 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China

关键词： FMD-inactivated vaccine; porcine circovirus type 2 infection; oxidative stress; CVC1302; immune efficacy

期刊名称：VIRAL IMMUNOLOGY （ 影响因子：1.695； 五年影响因子：1.528 ）

ISSN： 0882-8245

年卷期：

页码：

收录情况： SCI

摘要： This study aimed to explore the effect of the immunopotentiator CVC1302 on foot-and-mouth disease (FMD) vaccination in animals placed under oxidative stress. We established oxidative stress models using porcine circovirus type 2 (PCV2)-infected PK-15 cells and mice model both in vitro and in vivo, respectively. The efficacy of CVC1302 on PK-15 cells or in addition to the FMD vaccine was evaluated by quantitative real-time polymerase chain reaction, histopathological and enzyme-linked immunosorbent assay (ELISA) analysis. CVC1302 affected apoptosis of PCV2-infected PK-15 cells and significantly inhibited PCV2 replication, while it had no effect on the viability for blank PK-15 cell in vitro test with varying dilutions of CVC1302. Results showed that PCV2 induced a strong oxidative stress response in mice. CVC1302 reduced the viral load in spleen of PCV2-infected mice and ameliorated the pathological injury of spleen. Furthermore, CVC1302 significantly increased IgG antibody titer, cytokine expression, superoxide dismutase activity, catalase concentrations, and glutathione content in mice immunized with FMD vaccine. In conclusion, CVC1302 inhibits PCV2 replication and regulates oxidative stress in PCV2-infected mice, which can improve the immune efficacy of the FMD vaccine, providing a safe and effective immune enhancement.

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