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Promoting immunity with novel targeting antigen delivery vehicle based on bispecific nanobody

文献类型： 外文期刊

作者： Cheng, Haiwei 1 ; Yang, Li 1 ; Hou, Liting 1 ; Cai, Zizheng 5 ; Yu, Xiaoming 1 ; Du, Luping 1 ; Chen, Jin 1 ; Zheng, Qisheng 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Nanjing 210014, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China

5.Nanjing Agr Univ, Nanjing 210095, Peoples R China

关键词： Dendritic cell; Endocytosis; Bispecific nanobody; Vaccine; Targeted delivery

期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：5.6； 五年影响因子：5.6 ）

ISSN： 1567-5769

年卷期： 2023 年 119 卷

页码：

收录情况： SCI

摘要： As the most potent professional antigen presenting cells, dendritic cells (DCs) have been targeted in strategies to enhance vaccination efficacy. To date, targeted delivery has been mainly used for cancer therapy, with few studies focusing on vaccine antigens for animal epidemic diseases. In this study, we selected a series of mouse DC-specific nanobodies from a non-immunized camel. The four candidate nanobodies identified (Nb4, Nb13, Nb17, and Nb25), which showed efficient endocytosis of bone marrow-derived DCs, were evaluated as potential vaccine antigen targeted delivery vehicles. First, green fluorescent protein (GFP) was selected and four corre-sponding DCNb-GFP fusions were constructed for verification. Nb17-GFP was effective at promoting antibody production, inducing a cellular immune response, and increasing the IL-4 level. Second, foot-and-mouth disease virus (FMDV) and a FMDV-specific nanobody (Nb205) were selected and four bispecific nanobody DCNb-Nb205 fusions were generated to investigate the feasibility of a novel targeting antigen delivery vehicle. The resulting bispecific nanobody, Nb17-Nb205, could not only deliver FMDV particles instead of antigenic peptide, but also induced the production of specific antibodies, a cellular immune response, and IFN-gamma and IL-4 levels upon im-munization with a single subcutaneous injection. In conclusion, our results demonstrate the potential of bispe-cific nanobody as a novel and efficient DC-specific antigen delivery vehicle. This highlights the potential to expand targeted delivery to the field of animal epidemic diseases and provides a reference for the general application of nanotechnology in viral diseases.

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