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Molecular characterization and functional analysis of duck CCCH-type zinc finger antiviral protein (ZAP)

文献类型： 外文期刊

作者： Zhang, Rongrong 1 ; He, Yan 1 ; Zhu, Xinyu 3 ; Wen, Guoyuan 1 ; Luo, Qingping 1 ; Zhang, Tengfei 1 ; Lu, Qin 1 ; Liu, Shu 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Minist Agr & Rural Affairs, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Prov Key Lab Anim Pathogen Microbiol, Wuhan 430064, Peoples R China

3.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

关键词： Duck; ZAP; Interferon-beta; DTMUV

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：2.985； 五年影响因子：2.75 ）

ISSN： 0006-291X

年卷期： 2021 年 561 卷

页码：

收录情况： SCI

摘要： This is the first study to clone duck CCCH-type zinc finger antiviral protein (duZAP) from Jingjiang duck (Anas platyrhynchos). Full-length duZAP cDNA was 2154 bp and encoded a 717-amino acid polypeptide containing four highly conserved CCCH-type finger motifs, a WWE domain and a poly (ADP-ribose) polymerase (PARP) domain. duZAP was expressed in multiple duck tissues, with the highest mRNA expression in the spleen. Overexpression of duZAP in duck embryo fibroblast cells (DEFs) led to activation of the transcription factors IRF1 and NF-kappa B, and induction of IFN-beta. Analysis of deletion mutants revealed that both the WWE and PARP domains of duZAP were essential for activating the IFN-beta promoter. Knockdown of duZAP in DEFs significantly reduced poly (I:C)- and duck Tembusu virus (DTMUV)induced IFN-beta activation. Our findings further the understanding of the role of duZAP in the duck innate immune response. (C) 2021 Elsevier Inc. All rights reserved.

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