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Genome Features of Asaia sp. W12 Isolated from the Mosquito Anopheles stephensi Reveal Symbiotic Traits

文献类型: 外文期刊

作者: Chen, Shicheng 1 ; Yu, Ting 2 ; Terrapon, Nicolas 3 ; Henrissat, Bernard 3 ; Walker, Edward D. 6 ;

作者机构: 1.Oakland Univ, Sch Hlth Sci, Dept Clin & Diagnost Sci, 433 Meadowbrook Rd, Rochester, MI 48309 USA

2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Peoples R China

3.Aix Marseille Univ AMU, Ctr Natl Rech Sci CNRS, Architecture & Fonct Macromol Biol, UMR 7257, F-13288 Marseille, France

4.Inst Natl Rech Agron INRA, USC AFMB, F-1408 Marseille, France

5.King Abdulaziz Univ, Dept Biol Sci, Jeddah 21412, Saudi Arabia

6.Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

关键词: Asaia; paratransgenesis; symbiotic traits; Anopheles stephensi; genome features

期刊名称:GENES ( 影响因子:3.759; 五年影响因子:3.822 )

ISSN:

年卷期: 2021 年 12 卷 5 期

页码:

收录情况: SCI

摘要: Asaia bacteria commonly comprise part of the microbiome of many mosquito species in the genera Anopheles and Aedes, including important vectors of infectious agents. Their close association with multiple organs and tissues of their mosquito hosts enhances the potential for paratransgenesis for the delivery of antimalaria or antivirus effectors. The molecular mechanisms involved in the interactions between Asaia and mosquito hosts, as well as Asaia and other bacterial members of the mosquito microbiome, remain underexplored. Here, we determined the genome sequence of Asaia strain W12 isolated from Anopheles stephensi mosquitoes, compared it to other Asaia species associated with plants or insects, and investigated the properties of the bacteria relevant to their symbiosis with mosquitoes. The assembled genome of strain W12 had a size of 3.94 MB, the largest among Asaia spp. studied so far. At least 3585 coding sequences were predicted. Insect-associated Asaia carried more glycoside hydrolase (GH)-encoding genes than those isolated from plants, showing their high plant biomass-degrading capacity in the insect gut. W12 had the most predicted regulatory protein components comparatively among the selected Asaia, indicating its capacity to adapt to frequent environmental changes in the mosquito gut. Two complete operons encoding cytochrome bo(3)-type ubiquinol terminal oxidases (cyoABCD-1 and cyoABCD-2) were found in most Asaia genomes, possibly offering alternative terminal oxidases and allowing the flexible transition of respiratory pathways. Genes involved in the production of 2,3-butandiol and inositol have been found in Asaia sp. W12, possibly contributing to biofilm formation and stress tolerance.

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