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Elizabethkingia anophelis: Physiologic and Transcriptomic Responses to Iron Stress

文献类型： 外文期刊

作者： Chen, Shicheng 1 ; Johnson, Benjamin K. 1 ; Yu, Ting 2 ; Nelson, Brooke N. 1 ; Walker, Edward D. 1 ;

作者机构： 1.Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou, Peoples R China

3.Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA

关键词： Elizabethkingia; mosquito microbiota; iron; transcriptomics and genetics; physiology; oxidative stress

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： In this study, we investigated the global gene expression responses of Elizabethkingia anophelis to iron fluxes in the midgut of female Anopheles stephensi mosquitoes fed sucrose or blood, and in iron-poor or iron-rich culture conditions. Of 3,686 transcripts revealed by RNAseq technology, 218 were upregulated while 112 were down-regulated under iron-poor conditions. Hemolysin gene expression was significantly repressed when cells were grown under iron-rich or high temperature (37 degrees C) conditions. Furthermore, hemolysin gene expression was down-regulated after a blood meal, indicating that E. anophelis cells responded to excess iron and its associated physiological stress by limiting iron loading. By contrast, genes encoding respiratory chain proteins were up-regulated under iron-rich conditions, allowing these iron-containing proteins to chelate intracellular free iron. In vivo studies showed that growth of E. anophelis cells increased 3-fold in blood-fed mosquitoes over those in sucrose-fed ones. Deletion of siderophore synthesis genes led to impaired cell growth in both iron-rich and iron-poor media. Mutants showed more susceptibility to H2O2 toxicity and less biofilm formation than did wild-type cells. Mosquitoes with E. anophelis experimentally colonized in their guts produced more eggs than did those treated with erythromycin or left unmanipulated, as controls. Results reveal that E. anophelis bacteria respond to varying iron concentration in the mosquito gut, harvest iron while fending off iron-associated stress, contribute to lysis of red blood cells, and positively influence mosquito host fecundity.

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